Metagenomics: Methods and Protocols

Book Overview

Preface...
Table of Contents...
Contributing Authors...

1. Construction of Small-Insert and Large-Insert Metagenomic Libraries
Carola Simon and Rolf Daniel
2. Extraction of Total DNA and RNA from Marine Filter Samples and Generation of a Universal cDNA as Universal Template for Marker Gene Studies
Dominik Schneider, Franziska Wemheuer, Birgit Pfeiffer, and Bernd Wemheuer

3. Construction and Screening Marine Metagenomic Large Insert Libraries
Nancy Weiland-Br?uer, Daniela Langfeldt, and Ruth A. Schmitz

4. Constructing and Screening a Metagenomic Library of a Cold and Alkaline Extreme Environment
Mikkel A. Glaring, Jan K. Vester, and Peter Stougaard

5. DNA-, RNA-, and Protein-Based Stable-Isotope Probing for High-Throughput Biomarker Analysis of Active Microorganisms
Eleanor Jameson, Martin Taubert, Sara Coyotzi, Yin Chen, ?zge Eyice, Hendrik Sch?fer, J. Colin Murrell, Josh D. Neufeld, and Marc G. Dumont

6. Assessing Bacterial and Fungal Diversity in the Plant's Endosphere
Bernd Wemheuer and Franziska Wemheuer

7. Shotgun Metagenomic Sequencing analysis of Soft-Rot Enterobacteriaceae in Polymicrobial Communities
James Doonan, Sandra Denman, James E. McDonald, and Peter M. Golyshin

8. Cloning and Expression of Metagenomic DNA in Streptomyces lividans and its Subsequent Fermentation for Optimized Production
Yuriy Rebets, Jan Kormanec, Andriy Lutzhetskyy, Kristel Bernaerts, and Jozef Ann?

9. Degradation Network Reconstruction Guided by Metagenomic Data
Rafael Bargiela and Manuel Ferrer

10. Novel Tools for the Functional Expression of Metagenomics DNA
Nadine Katzke, Andreas Knapp, Anita Loeschcke, Thomas Drepper, and Karl-Erick Jaeger

11. A Microtiter Plate-Based Assay to Screen for Active and Stereoselective Hydrolytic Enzymes in Enzyme Libraries
Dominique B?ttcher, Patrick Z?gel, Marlen Schmidt, and Uwe T. Bornscheuer

12. Screening for Cellulase Encoding Clones in Metagenomics Libraries
Nele Ilmberger and Wolfgang R. Streit

13. Liquid Phase Multiplex High-Throughput Screening of Metagenomic Libraries using p-Nitrophenyl-Linked Substrates for Accessory Lignocellulosic Enzymes
Mariette Smart, Robert J. Huddy, Don A. Cowan, and Marla Trindade

14. Screening Glycosyltransferases for Polyphenol Modifications
Nele Ilmberger and Ulrich Rabausch

15. Methods for the Isolation of Genes Encoding Novel PHA Matabolism Enzymes from Complex Microbial CommunitiesJiujun Cheng, Ricardo Nordeste, Maria A. Trainer, and Trevor C. Charles

16. Function-Based Metagenomic Library Screening and Heterologous Expression Strategy for Genes Encoding Phosphatase Activity
Genis A. Castillo Villamizar, Heiko Nacke, and Rolf Daniel

17. Activity-Based Screening of Metagenomics Libraries for Hydrogenase Enzymes
Nicole Adam and Mirjam Perner

18. Screening for N-AHSL-Based-Signaling Interfering Enzymes
St?phane Uroz and Phil M. Oger

19. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites
F. Jerry Reen, Jose A. Guti?rrez-Barranquero, and Fergal O'Gara

• Format: Hardcover
• Language: English
• ISBN: 1493966898
• ISBN13: 9781493966899
• Release Date: November 2016
• Publisher: Humana
• Length: 303 Pages
• Weight: 1.70 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"