Mass Spectrometry Imaging: Principles and Protocols

By Stanislav S. Rubakhin

Book Overview

Introductory Reviews and Tutorials.- Imaging Mass Spectrometry: Viewing the Future.- A Mass Spectrometry Primer for Mass Spectrometry Imaging.- Imaging of Metals, Metalloids, and Non-metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in Biological Tissues.- Protocols for MS Imaging of Distribution of Small Molecules Including Metabolites and Pharmaceuticals.- Lipid Detection, Identification, and Imaging Single Cells with SIMS.- The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids.- Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Stores in Renal Epithelial Cells.- Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System.- Mapping Pharmaceuticals in Rat Brain Sections Using MALDI Imaging Mass Spectrometry.- Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry.- Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol.- Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS.- Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue.- Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry.- Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS).- Preparation of Single Cells for Imaging Mass Spectrometry.- Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Types.- Protocols for MS Imaging of Distribution of Peptides and Proteins.- Direct Molecular Analysis of Whole-Body Animal Tissue Sections by MALDI Imaging Mass Spectrometry.- MALDI Direct Analysis and Imaging of Frozen Versus FFPE Tissues: What Strategy for Which Sample?.- On Tissue Protein Identification Improvement by N-Terminal Peptide Derivatization.- Specific MALDI-MSI: TAG-MASS.- Structurally Selective Imaging Mass Spectrometry by Imaging Ion Mobility-Mass Spectrometry.- Tutorial: Multivariate Statistical Treatment of Imaging Data for Clinical Biomarker Discovery.- Applications of MALDI-MSI to Pharmaceutical Research.- Tissue Preparation for the In Situ MALDI MS Imaging of Proteins, Lipids, and Small Molecules at Cellular Resolution.- Imaging of Similar Mass Neuropeptides in Neuronal Tissue by Enhanced Resolution MALDI MS with an Ion Trap - OrbitrapTM Hybrid Instrument.- Mass Spectrometric Imaging of Neuropeptides in Decapod Crustacean Neuronal Tissues.- Mass Spectrometry Imaging Using the Stretched Sample Approach.

• Format: Paperback
• Language: English
• ISBN: 1617796964
• ISBN13: 9781617796968
• Release Date: October 2012
• Publisher: Humana
• Length: 490 Pages
• Weight: 1.75 lbs.
• Dimensions: 1.0" x 6.7" x 9.6"