Live Cell Imaging: Methods and Protocols

Book Overview

Part I: Imaging with Passive Optical Readouts

1. Fluorescent Labeling of the Nuclear Envelope without Relying on Inner Nuclear Membrane Proteins

Toshiyuki Taniyama and Shinji Sueda

2. Quantitative Analysis of Membrane Receptor Trafficking Manipulated by Optogenetic Tools

Osamu Takenouchi, Hideaki Yoshimura, and Takeaki Ozawa

3. Simultaneous Detection of Four Cell Cycle Phases with Live Fluorescence Imaging

Bryce T. Bajar and Michael Z. Lin

4. A Murine Bone Metastasis Model Using Caudal Artery Injection and Bioluminescence Imaging

Takahiro Kuchimaru and Shinae Kizaka-Kondoh

5. A New Lineage of Artificial Luciferases for Mammalian Cell Imaging

Sung-Bae Kim and Rika Fujii

6. Use of Bacterial Luciferase as a Reporter Gene in Eukaryotic Systems

Jittima Phonbuppha, Ruchanok Tinikul, and Pimchai Chaiyen

Part II: Imaging with Activatable Bioluminescent Probes

7. Quantitative Determination and Imaging of Gαq Signaling in Live Cells via Split-Luciferase Complementation

Timo Littmann, Takeaki Ozawa, and G?nther Bernhardt

8. A Split-Luciferase-Based Cell Fusion Assay for Evaluating the Myogenesis-Promoting Effects of Bioactive Molecules

Qiaojing Li, Hideaki Yoshimura, and Takeaki Ozawa

9. Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semi-Rational Molecular Design and Molecular Evolution

Marie Mita, Devina Wongso, Hiroshi Ueda, Takashi Tsuboi, and Tetsuya Kitaguchi

Part III: Imaging with Functional Substrates and Luciferases

10. Near Infrared Bioluminescence Imaging of Animal Cells with Through-Bond Energy Transfer Cassette

Masahiro Abe, Ryo Nishihara, Sung Bae Kim, and Koji Suzuki

11. Azide- and Dye-Conjugated Coelenterazine Analogues for Imaging Mammalian Cells

Ryo Nishihara, Emi Hoshino, Yoshiki Kakudate, Koji Suzuki, and Sung-Bae Kim

12. Luciferase-Specific Coelenterazine Analogues for Optical Crosstalk-Free Bioassays

Ryo Nishihara, Masahiro Abe, Koji Suzuki, and Sung-Bae Kim

Part IV: Imaging with Organic Fluorescent Probes

13. Live Imaging of Virus-Infected Cells by Using a Sialidase Fluorogenic Probe

Tadanobu Takahashi, Yuuki Kurebayashi, Tadamune Otsubo, Kiyoshi Ikeda, Akira Minami, and Takashi Suzuki

14. Live-Cell Imaging of Sirtuin Activity Using a One-Step Fluorescence Probe

Mitsuyasu Kawaguchi and Hidehiko Nakagawa

15. Live-Cell Fluorescence Imaging of Microtubules by Using a Tau-Deriv

• Format: Paperback
• Language: English
• ISBN: 1071612603
• ISBN13: 9781071612606
• Release Date: May 2022
• Publisher: Humana
• Length: 447 Pages
• Weight: 1.76 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"