Lipoproteins and Cardiovascular Disease: Methods and Protocols

Book Overview

Part I: RNA and Gene Expression

1. Cloning Full-Length Transcripts and Transcript Variants Using 5' and 3' RACE

Lita A. Freeman

2. Monitoring Gene Expression: Quantitative Real-Time RT-PCR

Elke M. Wagner

3. Microarray Technology: Basic Methodology and Application in Clinical Research for Biomarker Discovery in Vascular Diseases

Nalini Raghavachari

4. Northern Analysis of Gene Expression

Lita A. Freeman

5. Laser Capture Microdissection for Analysis of Macrophage Gene Expression from Atherosclerotic Lesions

Jonathan E. Feig and Edward A. Fisher

Part II: Sequencing

6. Sequencing PCR-Amplified DNA in Lipoprotein and Cardiovascular Disease Research

Victoria Youngblood and James G. Taylor VI

7. Introduction to Next-Generation Nucleic Acid Sequencing in Cardiovascular Disease Research

Lena Diaw, Victoria Youngblood, and James G. Taylor VI

Part III: Transgenic, Knockout, and Knockdown Methodologies

8. Strategies for Designing Transgenic DNA Constructs

Chengyu Liu

9. Purification of Plasmid and BAC Transgenic DNA Constructs

Chengyu Liu, Yubin Du, Wen Xie, and Changyun Gui

10. Pronuclear Microinjection and Oviduct Transfer Procedures for Transgenic Mouse Production

Chengyu Liu, Wen Xie, Changyun Gui, and Yubin Du

11. Genotyping of Transgenic Animals by Real-Time Quantitative PCR with TaqMan Probes

Boris Vaisman

12. Generation of General and Tissue-Specific Gene Knockout Mouse Models

Xian-Cheng Jiang

13. Adeno-Associated Viruses as Liver-Directed Gene Delivery Vehicles: Focus on Lipoprotein Metabolism

William R. Lagor, Julie C. Johnston, Martin Lock, Luk H. Vandenberghe, and Daniel J. Rader

14. Modulation of Lipoprotein Metabolism by Antisense Technology: Preclinical Drug Discovery Methodology

Rosanne M. Crooke and Mark J. Graham

Part IV: Special Topics

15. Chromatin Immunoprecipitation

Grant D. Barish and Rajenda K. Tangirala

16. Measurement of Lecithin: Cholesterol Acyltransferase Activity with the Use of a Peptide-Proteoliposome Substrate

Boris Vaisman and Alan T. Remaley

17. Native-Native 2D Gel Electrophoresis for HDL Subpopulation Analysis

Lita A. Freeman

18. Western Blots

Lita A. Freeman

• Format: Paperback
• Language: English
• ISBN: 1493956906
• ISBN13: 9781493956906
• Release Date: August 2016
• Publisher: Humana
• Length: 391 Pages
• Weight: 1.54 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"