Leukemia Stem Cells: Methods and Protocols

Book Overview

Part I: Introductory Reviews

1. Introduction and Classification of Leukemias

Geoffrey Brown

2. Leukemia Stem Cells: Concept and Implications

Isidro S?nchez-Garc?a and C?sar Cobaleda

3. Leukemia Stem Cell Drug Discovery

C?sar Cobaleda and Isidro S?nchez-Garc?a

Part II: Protocols

4. Analysis and Isolation of Mouse Leukemic Stem Cells

Fang Dong, Haitao Bai, and Hideo Ema

5. Mass Cytometry of Hematopoietic Cells

Astraea Jager, Jolanda Sarno, and Kara Davis

6. PCR Technology to Identify Minimal Residual Disease

Giovanni Cazzaniga, Simona Songia, and Andrea Biondi, on behalf of the EuroMRD Working Group

7. Next-Generation Sequencing Technology to Identify Minimal Residual Disease in Lymphoid Malignancies

Michaela Kotrova, Nikos Darzentas, Christiane Pott, and Monika Br?ggemann, on behalf of the EuroClonality-NGS Working Group

8. Genomic Inverse PCR for Screening of Preleukemic Cells in Newborns (GIPFEL Technology)

Daniel Hein, Arndt Borkhardt, and Ute Fischer

9. Single-Cell Transcriptomic Analysis of Hematopoietic Cells

Paulina M. Strzelecka, Anna M. Ranzoni, and Ana Cvejic

10. In-Depth Mass Spectrometry-Based Single-Cell and Nanoscale Proteomics

Yiran Liang, Thy Truong, Ying Zhu, and Ryan T. Kelly

11. In Vivo Clonal Analysis of Aged Hematopoietic Stem Cells: Single-Cell Transplantation

Kyomi J. Igarashi and Ryo Yamamoto

12. Experimental Competitive Bone Marrow Transplant Assays

Roxann H?tu-Arbour, Sarah Bouali, and Krista M. Heinonen

13. Human T-ALL Xenografts

Patricia Fuentes, Mar?a L. Toribio, and Sara Gonz?lez-Garc?a

14. An Experimental and Computational Protocol to Study Cell Proliferation in Human Acute Myeloid Leukemia Xenografts

Thalia Vlachou, Marco S. Nobile, Chiara Ronchini, Daniela Besozzi, and Pier Giuseppe Pelicci

15. Leukemic Stem Cell Culture in Cytokine-Free Medium

Xiaolei Liu and Peter S. Klein

16. Ex Vivo Expansion of Adult Hematopoietic Stem and Progenitor Cells with Valproic Acid

Luena Papa, Mansour Djedaini, Manisha Kintali, Christoph Schaniel, and Ronald Hoffman

17. Isolation, Culture, and Manipulation of Human Cord Blood Progenitors

Cristina Priet

• Format: Hardcover
• Language: English
• ISBN: 1071608096
• ISBN13: 9781071608098
• Release Date: November 2020
• Publisher: Humana
• Length: 479 Pages
• Weight: 2.30 lbs.
• Dimensions: 1.1" x 7.7" x 10.3"