Laser Capture Microdissection: Methods and Protocols

Book Overview

1. Laser Capture Microdissection: Insights into Methods and Applications

Meera Mahalingam

2. Laser Microdissection-Based Microproteomics of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues

R?mi Longuesp?e, Dominique Baiwir, Gabriel Mazzucchelli, Nicolas Smargiasso, and Edwin De Pauw

3. Isolation of DNA and RNA from Laser Microdissected Tissue Samples

Yelena G. Golubeva and Andrew C. Warner

4. Protocol for the Analysis of Laser Capture Microdissected Fresh-Frozen Tissue Homogenates by Silver-Stained 1D SDS-PAGE

DaRue A. Prieto, Gordon Whitely, Donald J. Johann, Jr., and Josip Blonder

5. Next Generation Sequencing Analysis of Laser-Microdissected Formalin-Fixed and Paraffin-Embedded (FFPE) Tissue Specimens

Lavinia M?gel, Stephan Bartels, and Ulrich Lehmann

6. Adaptation of Laser Microdissection Technique to Nanostring RNA Analysis in the Study of a Spontaneous Metastatic Mammary Carcinoma Mouse Model

Nadia P. Castro and Yelena G. Golubeva

7. Laser Capture Microdissection as a Tool to Study the Mucosal Immune Response in Celiac Disease

Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, and Giuseppe Mazzarella

8. Laser Capture Microdissection and Isolation of High Quality RNA from Frozen Endometrial Tissue

Michele Cummings, Georgia Mappa, and Nicolas M. Orsi

9. Laser Microdissection for Human Papillomavirus (HPV) Genotyping Attribution and Methylation Pattern Analyses of Squamous Intraepithelial Lesions

Monica Molano, Suzanne M. Garland, and Alyssa M. Cornall

10. Laser Capture Microdissection and Transcriptional Analysis of Sub-Populations of the Osteoblast Lineage from Undecalcified Bone

Efrain Pacheco, Rong Hu, and Scott Taylor

11. Cell Type-Specific Laser Capture Microdissection for Gene Expression Profiling in the Human Brain

Sarah A. Mauney, Tsung-Ung W. Woo, and Kai C. Sonntag

12. The Isolation of Pure Populations of Neurons by Laser Capture Microdissection: Methods and Application in Neuroscience

Ren?e Morris and Prachi Mehta

13. Laser Capture Microdissection in Traumatic Brain Injury Research: Obtaining Hippocampal Subregions and Pools of Injured Neurons for Genomic Analyses

Deborah R. Boone, Harris A. Weisz, Stacy L. Sell, and Helen L. Hellmich

14. Isolation of Distinct Types of Neurons from Fresh Brain Tissue Using Laser Microdissection in Combination with High Performance Liquid Chromatography - Mass Spectrometry

Luisa Aring, Simone Steinbach, Katrin Marcus, and Caroline May

15. Immuno-Guided Laser Capture Microdissection of Glial Cells for mRNA Analysis

Arnaud B. Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, and David A. Laplaud

16. Immuno-Laser Capture Microdissection for the Isolation of Enriched Glial Populations from Frozen Post Mortem Human Brain

Julie E. Simpson, Stephen B. Wharton, and Paul R. Heath

17. Laser Capture Microdissection for the Analysis of Rat and Human Spinal Cord Ependyma by qPCR

Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, and Eduardo Molina-Holgado

18. Isolation of Amyloid Plaques and Neurofibrillary Tangles from Archived Alzheimer's Disease Tissue Using Laser Capture Microdissection for Downstream Proteomics

Eleanor Drummond, Shruti Nayak, Geoffrey Pires, Beatrix Ueberheide, and Thomas Wisniewski

19. Cell-Specific RNA Quantification in Human SN DA Neurons from Heterogeneous Post Mortem Midbrain Samples by UV-

• Format: Hardcover
• Language: English
• ISBN: 1493975579
• ISBN13: 9781493975570
• Release Date: January 2018
• Publisher: Humana
• Length: 412 Pages
• Weight: 2.11 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"