Isolation of Plant Organelles and Structures: Methods and Protocols

Book Overview

1. The Isolation of Plant Organelles and Structures in the Post-Genomics Era

A. Harvey Millar and Nicolas L. Taylor

2. Approaches to Characterize Organelle, Compartment, or Structure Purity

Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski

Part I: Isolation of Organelles and Organelle Compartments

3. Isolation of Nuclei and Nucleoli

Alison F. Pendle and Peter J. Shaw

4. Isolation and Suborganellar Fractionation of Arabidopsis Chloroplasts

?rsula Flores-P?rez and Paul Jarvis

5. Isolation of Chromoplasts and Sub-Organellar Compartments from Tomato and Bell Pepper Fruit

Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech

6. Leucoplast Isolation and Subfractionation

William C. Plaxton

7. Isolation of Mitochondria, Their Sub-Organellar Compartments, and Membranes

Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor

8. Isolation of Arabidopsis Leaf Peroxisomes and the Peroxisomal Membrane

Sigrun Reumann and Piotr Lisik

9. Isolation of Vacuoles and the Tonoplast

Jan Zouhar

10. Isolation of Endoplasmic Reticulum and Its Membrane

G. Eric Schaller

11. Enrichment of Golgi Membranes from Triticum aestivum (Wheat) Seedlings

Wei Zeng, Berit Ebert, Harriet T. Parsons, Carsten Rautengarten, Antony Bacic, and Joshua L. Heazlewood

12. Isolation of Autolysosomes from Tobacco BY-2 Cells

Chihiro Takatsuka, Yuko Inoue, and Yuji Moriyasu

13. Isolation of Protein Storage Vacuoles and Their Membranes

Tomoo Shimada and Ikuko Hara-Nishimura

Part II: Isolation of Other Structures and Compartments

14. Isolation of the Cell Wall

Herv? Canut, C?cile Albenne, and Elisabeth Jamet

15. Isolation of Plasmodesmata

Christine Faulkner and Emmanuelle M.F. Bayer

16. Isolation of Plasma Membrane and Plasma Membrane Microdomains

Anzu Minami, Daisuke Takahashi, Yukio Kawamura, and Matsuo Uemura

17. Enrichment of the Plant Cytosolic Fraction

Jeemeng Lao, Andreia M. Smith-Moritz, Jennifer C. Mortimer, and Joshua L. Heazlewood

18. Isolation of Apoplast

Erik Andreasson, Kibrom B. Abreha, and Svante Resj

• Format: Paperback
• Language: English
• ISBN: 1493982281
• ISBN13: 9781493982288
• Release Date: June 2018
• Publisher: Humana
• Length: 338 Pages
• Weight: 1.35 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"