Invariant Natural Killer T-Cells: Methods and Protocols

Book Overview

1. Genetic Analysis of iNKT Cell Development and Function

Julie Sahler, Orchi Anannya, Candice Limper, Brian Imbiakha, Tim Pierpont, and Avery August

2. Genetic Studies of Natural Glycosphingolipid Ligands for NKT Cells

Dapeng Zhou, Chengfeng Xia, Peng G. Wang, Zhiyuan Li, Wenpeng Zhang, Guanghui Ni, and Jiaxi Cheng

3. Retroviral Transduction of NKT Hybridoma Cells

Ke Wang, Rong Jin, and Qing Ge

4. Methods for Studying Mouse and Human Invariant Natural Killer T-Cells

Yang Zhou, Yan-Ruide Li, Samuel Zeng, and Lili Yang

5. Isolation and Detection of Murine iNKT Cells in Different Organs

Mengqing Cong, Xiang Li, Haopeng Fang, Li Bai, Xucai Zheng, and Bofeng Li

6. Identifying, Isolation, and Functional Use of Human Liver iNKT Cells

Wenjing He, Dongmei Ye, and Yifang Gao

7. Isolation and Characterization Methods of Human Invariant NKT Cells

Rui Liu and Hua Wang

8. Detection of Mouse Type I NKT (iNKT) Cells by Flow Cytometry

Vibhuti Joshi and Masaki Terabe

9. Identification of Rare Thymic NKT Cell Precursors by Multiparameter Flow Cytometry

Jihene Klibi and Kamel Benlagha

10. iNKT Cell Transfer: The Use of Cell Sorting Combined with Flow Cytometry Validation Approach

Marcella Cipelli, Theresa Ramalho, Cristhiane Favero de Aguiar, and Niels Olsen Saraiva Camara

11. Expansion of Human iNKT Cells Ex Vivo

Jing Wang, Chen Zhao, and Jianqing Xu

12. The Expansion and Cytotoxicity Detection of Human iNKT Cells

Xue Cheng, Xiaosheng Tan, Rui Dou, Xiongwen Wu, and Xiufang Weng

13. Ex Vivo Expansion of Th2-Polarizing Immunotherapeutic iNKT Cells from Human Peripheral Blood

Natasha K. Khatwani, Kelly J. Andrews, and Asha B. Pillai

14. Intravital Microscopy Imaging of Invariant Natural Killer T-Cell Dynamics in the Liver Using CXCR6-eGFP Transgenic Mice

Zhou Hong, Zeng Zhutian, and Wang Fei

15. In Vivo Cytotoxicity by α-GalCer-Transactivated NK Cells

Patrick T. Rudak and S.M. Mansour Haeryfar

16. Redirecting iNKT Cell Anti-Tumor Immunity with αGalCer/CD1d-scFv Fusion Proteins

Lianjun Zhang and Alena Donda

17. Investigating the Dynamic Changes in iNKT Cell Metabolic Profiles during Development

Jana L. Raynor and Hongbo Chi

• Format: Paperback
• Language: English
• ISBN: 107161777X
• ISBN13: 9781071617779
• Release Date: September 2022
• Publisher: Humana
• Length: 195 Pages
• Weight: 0.81 lbs.
• Dimensions: 0.4" x 7.0" x 10.0"