Immune Homeostasis: Methods and Protocols

Book Overview

1. Quantitating Lymphocyte Programmed Cell Death (PCD) In Vitro Using Simple Kill Assays

Lixin Zheng

2. Fluoresence-Activated Cell Sorting (FACS)-Based Quantitation of T Cell Receptor Restimulation-Induced Cell Death (RICD) in Activated, Primary Human T Cells

Gil Katz and Andrew L. Snow

3. Evaluation of IL-2-Withdrawal Induced Apoptosis in Human T Lymphocytes

Joao Bosco Oliveira

4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique

Bernice Lo and Madhu Ramaswamy

5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation

Nelia Cordeiro and Nicolas Bid?re

6. Analyses of Programmed Cell Death in Dendritic Cells

Min Chen, Lily Huang, and Jin Wang

7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity

Francis Ka-Ming Chan, Kenta Moriwaki, and Mar?a Jos? De Rosa

8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers

Sema Kurtulus and David Hildeman

9. Homeostatic Proliferation of Mature T Cells

Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh

10. Quantitating Lymphocyte Homeostasis In Vivo in Humans Using Stable Isotope Tracers

Liset Westera, Yan Zhang, Kiki Tesselaar, Jos? A.M. Borghans, and Derek C. Macallan

11. Real-Time Quantitative (RQ-)PCR Approach to Quantify the Contribution of Proliferation to B Lymphocyte Homeostasis

Menno C. van Zelm, Magdalena A. Berkowska, Mirjam van der Burg, and Jacques J.M. van Dongen

12. Molecular Measurement of T Cell Receptor Excision Circles

Heather E. Lynch and Gregory D. Sempowski

13. Isolation of RNA and the Synthesis and Amplification of cDNA from Antigen-Specific T Cells for Genome-Wide Expression Analysis

R. Anthony Barnitz, Sabrina Imam, Kathleen Yates, and W. Nicholas Haining

14. Designs for Massively Parallel Sequencing Approaches to Identify Causal Mutations in Human Immune Disorders

Yu Zhang and Helen C. Su

15. Flow Cytometric Measurement of SLAM-Associated Protein (SAP) and X-Linked Inhibitor of Apoptosis (XIAP)

Rebecca A. Marsh, Jack J. Bleesing, and Alexandra H. Filipovich

16. In Vitro Suppression Assay for Functional Assessment of Human Regulatory T Cells

Dat Q. Tran

4. Determination of Apoptosis Sensitivity in Specific T Cell Subsets from Human Peripheral Blood by Utilizing a Multi-Parameter Fluorescence Activated Cell Sorting (FACS)-Based Technique

Bernice Lo and Madhu Ramaswamy

5. Visualization of Fas-Mediated Death-Inducing Signaling Complex (DISC) Formation by Immunoprecipitation

Nelia Cordeiro and Nicolas Bid?re

6. Analyses of Programmed Cell Death in Dendritic Cells

Min Chen, Lily Huang, and Jin Wang

7. Detection of Necrosis by Release of Lactate Dehydrogenase (LDH) Activity

Francis Ka-Ming Chan, Kenta Moriwaki, and Mar?a Jos? De Rosa

8. Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I & II Tetramers

Sema Kurtulus and David Hildeman

9. Homeostatic Proliferation of Mature T Cells

Christopher E. Martin, Kwesi Frimpong-Boateng, Darina S. Spasova, John C. Stone, and Charles D. Surh

10. Quantitating Lymphocyte Homeostasis In Vivo in Humans U

• Format: Hardcover
• Language: English
• ISBN: 1627032894
• ISBN13: 9781627032896
• Release Date: February 2013
• Publisher: Humana
• Length: 215 Pages
• Weight: 1.25 lbs.
• Dimensions: 0.7" x 7.2" x 10.0"