The consumption of seafood has been increasing globally due to its nutritional benefits. However, it also poses a potential threat to individuals who suffer from food allergies, especially those who are allergic to shellfish. Mud crab, one of the popular shellfish, contains tropomyosin, an allergenic protein that can cause shellfish allergy. Therefore, it is crucial to identify and characterize tropomyosin in mud crab for effective diagnosis, treatment,...