Introduction to petroleum, hydrocarbon and lipid analysis.- Hydrocarbon extraction.- Determination of petroleum hydrocarbon compounds from soils and sediments using ultrasonic extraction.- Protocols for the chemical analysis of hydrocarbons in petroleum oils and the assessment of environmental contamination.- Analysis of lipids and polycyclic aromatic hydrocarbons as indicators of past and present (micro)biological activity- Protocols for GC-based methods for hydrocarbon analysis.- Comprehensive two dimensional gas chromatography to assess petroleum product weathering.- Measuring hydrocarbons in the atmosphere.- Protocols for the Quantification of Dimethyl Sulfide (DMS) and Other Volatile Organic Compounds in Aquatic Environments.- Fluorescence EEMs and PARAFAC techniques in the analysis of petroleum components in the water column.- Protocols for Measurement of Naphthenic Acids in Aqueous Samples.- Compositional Characterization of Acidic Petroleum Constituents Using Negative Ion Mode Electrospray Ionization Coupled with Fourier Transform-Ion Cyclotron Resonance Mass Spectrometry.- Measurement of hydrocarbon bioavailability in soil.- Measurements of hydrocarbon pollutants in aqueous samples using bioreporter assays.- Protocol to investigate volatile aromatic hydrocarbon degradation with purge and trap coupled to a gas-chromatograph / isotope ratio mass spectrometer.- Comprehensive analysis of microbial lipids in environmental samples through HPLC-MS protocols.