Human Embryonic Stem Cells Handbook

Book Overview

1. Establishment of New Lines of Human Embryonic Stem Cells: Evolution of the Methodology

Ana Maria Fraga, ?rica Sara Souza de Ara?jo, Raquel Stabellini, Naja Vergani, and Lygia V. Pereira

2. Successful Scale-Up and Quality Assessments of Human Embryonic Stem Cells for Cell Therapy: Challenges and Overview

Udaykumar Kolkundkar, Mohan Vemuri, Geetha.M.Swamilingiah, Shruthi Pal, and Jasmeet Kaur

3. Human Embryonic Stem Cells Derived in Xeno-Free Conditions

Amparo Gal?n Albi?ana and Carlos Sim?n Vall?s

4. Procedures for Derivation and Characterization of Human Embryonic Stem Cells from Odense, Denmark

Linda Harkness and Moustapha Kassem

5. Principles for Derivation of Human Embryonic Stem Cells

Mikael C.O. Englund, Catharina Ellerstr?m, Katarina Andersson, Karin Noaksson, and Johan Hyllner

6. Derivation and Maintenance of Undifferentiated Human Embryonic Stem Cells

Guoliang Meng and Derrick E. Rancourt

7. Establishment of hESC Lines from the Inner Cell Mass of Blastocyst-Stage Embryos and Single Blastomeres of 4-Cell Stage Embryos

Ileana Mateizel, Mieke Geens, Hilde Van de Velde, and Karen Sermon

8. Analysis of LINE-1 Expression in Human Pluripotent Cells

Martin Mu?oz-Lopez, Marta Garcia-Ca?adas, Angela Macia, Santiago Morell, and Jose L. Garcia-Perez

9. Characterization and Gene Expression Profiling of Five Human Embryonic Stem Cell Lines Derived in Taiwan

Steven Shoei-Lung Li

10. Derivation of Human Embryonic Stem Cell Lines from Poor Quality Embryos

Ronak Shetty and Maneesha S. Inamdar

11. Derivation, Expansion, and Characterization of Human Embryonic Stem Cell Lines from Aneuploid Embryos

Juan-Carlos Biancotti and Neta Lavon

12. Mutated Human Embryonic Stem Cells for the Study of Human Genetic Disorders

Ahmi Ben-Yehudah, Mira Malcov, Tsvia Frumkin, and Dalit Ben-Yosef

13. Single-Cell Enzymatic Dissociation of hESC Lines OxF1--OxF4 and Culture in Feeder-Free Conditions

Frances A. Brook

14. Protocol for Expansion of Undifferentiated Human Embryonic and Pluripotent Stem Cells in Suspension

Hossein Baharvand, Mehran Rezaei Larijani, and Maryam Yousefi

15. Suspension Bioreactor Expansion of Undifferentiated Human Embryonic Stem Cells

Roman Krawetz and Derrick E. Rancourt

16. Derivation, Propagation, and Characterization of Neuroprogenitors from Pluripotent Stem Cells (hESCs and hiPSCs)

Khun-Hong Lie, Henry Chung, and Kuldip S. Sidhu

17. Comparison of Neural Differentiation Potential of Human Pluripotent Stem Cell Lines Using a Quantitative Neural Differentiation Protocol

Dezhong Yin, Tahereh Tavakoli, Wei-Qiang Gao, and Wu Ma

18. Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells

Aaron M. Elliott, Kristi A. Hohenstein Elliott, and Anja Kammesheidt

19. Chromatin Immunoprecipitation-Based Analysis of Gene Regulatory Networks Operative in Human Embryonic Stem Cells

Marc Jung and James Adjaye

20. Analysis of the Methylome of Human Embryonic Stem Cells Employing Methylated DNA Immunoprecipitation Coupled to Next Generation Sequencing (MeDIP-seq)

Christina Grimm and James Adjaye

21. Stable Isotope Labe

• Format: Paperback
• Language: English
• ISBN: 1493962620
• ISBN13: 9781493962624
• Release Date: August 2016
• Publisher: Humana
• Length: 313 Pages
• Weight: 1.26 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"