How to Genotype Rotavirus Isolates

By Philip Anochie

Viral RNA was extracted from the stool samples collected from the children and analyzed using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR ) for genotyping and Agarose Gel Electrophoresis (AGE) for the identification of the different bands of the electrophoretypes of the rotavirus. Analysis results indicated that 16 (22.53%%) out of the 71 children were positive for rotavirus infection while 55 (77.46%%) were negative for rotavirus infection. The rotavirus positive patients were made up of 12 males (68.75%%) and 4 females (25.0%%) while rotavirus negative patients were 32 males (58.18%%) and 23 females (41.81%%). The total number of males with gastroenteritis were 44 (60.56%%) while females were 27 (39.43%%). Rotavirus G2 genotype detected in 10 patients was the most prevalent strain in the hospital followed by G1 genotype found in 6 patients and these were the only rotavirus genotypes detected in the hospital.

• Format: Paperback
• Language: English
• ISBN: 0359002056
• ISBN13: 9780359002054
• Release Date: August 2018
• Publisher: Lulu.com
• Length: 124 Pages
• Weight: 0.39 lbs.
• Dimensions: 0.3" x 6.0" x 9.0"