Host-Fungus Interactions: Methods and Protocols

Book Overview

Part I: Gene Disruption

1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy

Christoph Sasse and Joachim Morschh?user

2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans

Shantanu Ganguly and Aaron P. Mitchell

3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans

Megan D. Lenardon and Andr? Nantel

4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi

Olga Zemska and Chad A. Rappleye

5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers

Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn

6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System

Lorina G. Bakerand and Jennifer K. Lodge

7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast

Iran Malavazi and Gustavo Henrique Goldman

8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach

Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard

9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers

Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole

Part II: Modulation of Gene Expression: RNAi Gene Knockdown

10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum

Brian H. Youseff and Chad A. Rappleye

11. RNA Interference in Cryptococcus neoformans

Michael L. Skowyra and Tamara L. Doering

12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA

Jo?o F. Menino, Agostinho J. Almeida, and Fernando Rodrigues

Part III: Modulation of Gene Expression: Regulatable Promoters

13. Tetracycline-Inducible Gene Expression in Candida albicans

Michael Weyler and Joachim Morschh?user

14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii

Lorina G. Baker and Jennifer K. Lodge

15. Modular Gene Over-Expression Strategies for Candida albicans

Vitor Cabral, Murielle Chauvel, Arnaud Firon, M?lanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d'Enfert

Part IV: Responses to Infection In Vivo

16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans

H?ctor M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow

17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans

Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong

18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils

Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg

19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions

Torsten W?llert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom

• Format: Paperback
• Language: English
• ISBN: 1493959042
• ISBN13: 9781493959044
• Release Date: August 2016
• Publisher: Humana
• Length: 597 Pages
• Weight: 2.32 lbs.
• Dimensions: 1.2" x 7.0" x 10.0"