Glycosyltransferases: Methods and Protocols

Book Overview

1. Glycan Microarray Screening Assay for Glycosyltransferase Specificities

Wenjie Peng, Corwin M. Nycholat, and Nahid Razi

2. A Fluorescence-Based Assay for Core 1 3Galactosyltransferase (T-synthase) Activity

Tongzhong Ju and Richard D. Cummings

3. Structural and Biochemical Analysis of a Bacterial Glycosyltransferase

Fan Zhu, Ren Wu, Hua Zhang, and Hui Wu

4. Study of the Biological Functions of Mucin Type Core 3 O-glycans

Seung Ho Lee and Minoru Fukuda

5. Generation of Anti-Sulfated Glycan Antibodies using Sulfotransferase-deficient Mice

Hiroto Kawashima

6. Fluorescent Microscopy as a Tool to Elucidate Dysfunction and Mislocalization of Golgi Glycosyltransferases in COG Complex Depleted Mammalian Cells

Rose A. Willett, Irina D. Pokrovskaya, and Vladimir V. Lupashin

7. A Practical Approach to Reconstruct Evolutionary History of Animal Sialyltransferases and Gain Insights into the Sequence-Function Relationships of Golgi-Glycosyltransferases

Daniel Petit, Roxana Elin Teppa, Jean-Michel Petit, and Anne Harduin-Lepers

8. Fluorescent Lectin Staining of Drosophila Embryos and Tissues to Detect the Spatial Distribution of Glycans during Development

E. Tian, Liping Zhang, and Kelly G. Ten Hagen

9. Photoaffinity Labeling of Protein O-Mannosyltransferases of the PMT1/PMT2 Subfamily

Martin Loibl and Sabine Strahl

10. Enzymatic Analysis of the Protein O-glycosyltransferase, Rumi, Acting toward Epidermal Growth Factor-like (EGF) Repeats

Hideyuki Takeuchi and Robert S. Haltiwanger

11. Enzymatic Characterization of Recombinant Enzymes of O-GlcNAc Cycling

Eun Ju Kim and John Hanover

12. Antibodies and Activity Measurements for the Detection of O-GlcNAc Transferase, and Assay of its Substrate, UDP-GlcNAc

Tony Lefebvre, Ludivine Drougat, St?phanie Olivier-van Stichelen, Jean-Claude Michalski, and Anne-Sophie Vercoutter-Edouart

13. In vitro Glycosylation Assay for Bacterial Oligosaccharyltransferases

Matias A. Musumeci, Maria V. Ielmini, and Mario F. Feldman

14. In vitro UDP-sugar: Undecaprenyl-Phosphate Sugar-1-Phosphate Transferase Assay and Product Detection by Thin Layer Chromatography

Kinnari B. Patel and Miguel A. Valvano

15. In vitro O-Antigen Ligase Assay

Xiang Ruan and Miguel A. Valvano

16. Functional Identification of Bacterial Glucosyltransferase WbdN

Yin Gao, Anna Vinnikova, and Inka Brockhausen

17. Methods for the Pasteurella Glycosaminoglycan Synthases: Enzymes that Polymerize Hyaluronan, Chondroitin, or Heparosan Chains

Paul DeAngelis

18. Methods for Measuring Class I Membrane-bound Hyaluronan Synthase Activity

Paul H. Weigel, Amy J. Padgett-McCue, and Bruce A. Baggenstoss

19. Creation and Characterization of Glycosyltransferase Mutants of Trypanosoma brucei

Luis Izquierdo, M. Lucia S. G?ther, and Michael A.J. Ferguson

20. Mannose-6-Phosphate: A Regulator of LLO Destruction

Ningguo Gao and Mark A. Lehrman

21. N-Acetylglucosaminyltransferase (GnT) Assays using Fluorescent Oligosaccharide Acceptor Substrates: GnT-III, IV, V, and IX (GnT-Vb)

Shinji Takamatsu, Hiroaki Korekane, Kazuaki Ohtsubo, Suguru Oguri, Jong Yi Park, Akio Matsumoto, and Naoyuki Taniguchi

22. A Method for Determination of UDP-GlcNAc: GlcNAcβ1-6(GlcNAcβ1-2)Manα1-R [GlcNAc to Man] β1-4N-acetylglucosaminyltransferase VI Activity

Tomohiko Taguchi and Naoyuki Taniguchi

23. In vitro Assays of Orphan Glycosyltransferases and their Application to Identify Notch Xylosyltransferases

Maya K. Sethi, Falk F

• Format: Paperback
• Language: English
• ISBN: 1493959964
• ISBN13: 9781493959969
• Release Date: August 2016
• Publisher: Humana
• Length: 425 Pages
• Weight: 1.70 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"