Genomic Imprinting: Methods and Protocols

Book Overview

Part I: Parent-of-Origin Effects

1. Uniparental Embryos in the Study of Genomic Imprinting

Yong Cheng, Dasari Amarnath, and Keith E. Latham

2. Derivation of Induced Pluripotent Stem Cells by Retroviral Gene Transduction in Mammalian Species

Masanori Imamura, Hironobu Okuno, Ikuo Tomioka, Yoshimi Kawamura, Zachary Yu-Ching Lin, Ryusuke Nakajima, Wado Akamatsu, Hirotaka James Okano, Yumi Matsuzaki, Erika Sasaki, and Hideyuki Okano

3. Generation of Trophoblast Stem Cells

Michael C. Golding

4. Immunomagnetic Purification of Murine Primordial Germ Cells

Emily Y. Smith and James L. Resnick

Part II: Identifying Imprinted Genes

5. Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA

Andrew J. Sharp

6. Identification of Imprinted Loci by Transcriptome Sequencing

Tomas Babak

7. Data Mining as a Discovery Tool for Imprinted Genes

Chelsea Brideau and Paul Soloway

Part III: Identifying the Regulatory Features of Imprinted Domains

8. Engineering of Large Deletions and Duplications In Vivo

Louis Lefebvre

Part IV: Epigenetics of Imprinted Regions

9. Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells

Julie Borgel, Sylvain Guibert, and Michael Weber

10. Chromatin Immunoprecipitation to Characterize the Epigenetic Profiles of Imprinted Domains

Purnima Singh and Piroska E. Szab?

11. Quantitative Chromosome Conformation Capture

Raffaella Nativio, Yoko Ito, and Adele Murrell

12. Genome-Wide Analysis of DNA Methylation in Low Cell Numbers by Reduced Representation Bisulfite Sequencing

S?bastien A. Smallwood and Gavin Kelsey

Part V: Analysis of Imprinted Expression

13. Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies

Sarah Rose Huffman, Md Almamun, and Roc?o Melissa Rivera

14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

Mathieu Rederstorff

15. Co-Immunoprecipitation of Long Non-Coding RNAs

Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil

Part VI: Imprinting in Plants

16. Specialized Technologies for Epigenetics in Plants

Wenyan Xiao

Part VII: Evolution of Imprinted Genes

17. Computational Studies of Imprinted Genes

Martina Paulsen

18. Insights on Imprinting from Beyond Mice and Men

Andrew Pask

19. Non-Mammalian Parent-Of-Origin Effects

Elena de la Casa-Esper?n

14. Generation of cDNA Libraries from RNP-Derived Regulatory Non-Coding RNAs

Mathieu Rederstorff

15. Co-Immunoprecipitation of Long Non-Coding RNAs

Victoria A. Moran, Courtney N. Niland, and Ahmad M. Khalil

Part VI: Imprinting in Plants

16. Specialized Technologies for Epigenetics in Plants

Wenyan Xiao

Part VII: Evolution of Imprinted Genes

17. Computational Studies of Imprinted Genes

Martina

• Format: Paperback
• Language: English
• ISBN: 1493959662
• ISBN13: 9781493959662
• Release Date: August 2016
• Publisher: Humana
• Length: 297 Pages
• Weight: 1.20 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"