Microbial Gene Essentiality: Protocols and Bioinformatics

By Svetlana Y. Gerdes

Book Overview

This collection of experimental and computational strategies and techniques for microbial genome-scale essentiality studies was developed and written by the leading research groups in the field. In addition to wet-lab protocols, the book describes statistical methods essential for planning and evaluating successful large-scale essentiality screens; in-silico prediction of gene essentiality using genome-scale reconstructed metabolic models; and data integration and comparative analysis of genomic databases. Each protocol follows the successful Methods in Molecular Biology(TM) series format, offering an introduction outlining the underlying principles, step-by-step instructions, a list of necessary equipment and reagents, and tips on troubleshooting and avoiding pitfalls.

• Format: Paperback
• Language: English
• ISBN: 1617375292
• ISBN13: 9781617375293
• Release Date: November 2010
• Publisher: Humana
• Length: 486 Pages
• Weight: 1.53 lbs.
• Dimensions: 1.0" x 6.1" x 9.2"