G Protein-Coupled Receptor Signaling in Plants: Methods and Protocols

Book Overview

1. Topology Assessment, G Protein-Coupled Receptor (GPCR) Prediction, and In Vivo Interaction Assays to Identify Plant Candidate GPCRs

Timothy E. Gookin and Jannick D. Bendtsen

2. Measurement of GTP-Binding and GTPase Activity of Heterotrimeric Gα Proteins

Swarup Roy Choudhury, Corey S. Westfall, Dieter Hackenberg, and Sona Pandey

3. Biochemical Analysis of the Interaction Between Phospholipase Dα1 and GTP-Binding Protein α-Subunit from Arabidopsis thaliana

Jian Zhao and Xuemin Wang

4. Analysis of Cell Division and Cell Elongation in the Hypocotyls of Arabidopsis Heterotrimeric G Protein Mutants

Zhaoqing Jin, Wellington Muchero, and Jin-Gui Chen

5. Aequorin Luminescence-Based Functional Calcium Assay for Heterotrimeric G Proteins in Arabidopsis

Kiwamu Tanaka, Jeongmin Choi, and Gary Stacey

6. Methods for Analysis of Disease Resistance and the Defense Response in Arabidopsis

Guojing Li, Xiujuan Zhang, Dongli Wan, Shuqun Zhang, and Yiji Xia

7. Fusarium oxysporum Infection Assays in Arabidopsis

Yuri Trusov, David Chakravorty, and Jose Ramon Botella

8. Analysis of Unfolded Protein Response in Arabidopsis

Yani Chen and Federica Brandizzi

9. Functional Analysis of Heterotrimeric G Proteins in Chloroplast Development in Arabidopsis

Wenjuan Wu and Jirong Huang

10. G Protein Signaling in UV Protection: Methods for Understanding the Signals in Young Etiolated Seedlings

Danielle A. Orozco-Nunnelly, Lon S. Kaufman, and Katherine M. Warpeha

11. Functional Analysis of Small Rab GTPases in Cytokinesis in Arabidopsis thaliana

Xingyun Qi and Huanquan Zheng

12. In Vivo Localization in Arabidopsis Protoplasts and Root Tissue

Myoung Hui Lee, Yongjik Lee, and Inhwan Hwang

13. Analysis of Protein Prenylation and S-Acylation Using Gas Chromatography-Coupled Mass Spectrometry

Nadav Sorek, Amir Akerman, and Shaul Yalovsky

14. In Vitro Myristoylation Assay of Arabidopsis Proteins

Xuehui Feng, Wan Shi, Xuejun Wang, and Mark P. Running

15. Assaying Protein S-Acylation in Plants

Piers A. Hemsley

16. In Vitro Prenylation Assay of Arabidopsis Proteins

Wan Shi, Qin Zeng, and Mark P. Running

• Format: Paperback
• Language: English
• ISBN: 1493962167
• ISBN13: 9781493962167
• Release Date: August 2016
• Publisher: Humana
• Length: 162 Pages
• Weight: 0.69 lbs.
• Dimensions: 0.4" x 7.0" x 10.0"