Fluorescent Methods for Molecular Motors

Fluorescence and Labelling: How to choose and what to do.- Fluorescent biosensors: design and application to motor proteins.- Rapid Reaction Kinetic Techniques.- Fluorescence to study the ATPase mechanism of Motor Proteins.- Use of pyrene labelled actin to probe actin myosin interactions; kinetic and equilibrium studies.- Fluorescent methods to study transcription initiation and transition into elongation.- Single-molecule and single-particle imaging of molecular motors in vitro and in vivo.- Fluorescence methods in the investigation of the DEAD-box helicase mechanism.- Use of Fluorescent Techniques to Study the In Vitro Movement of Myosins.- Fluorescence Tracking of Motor proteins in vitro.- Measuring Transport of Motor Cargos.- Measuring two at the same time: Combining Magnetic Tweezers with Single-Molecule FRET.- Using fluorescence to study actomyosin in yeasts.

• Format: Hardcover
• Language: English
• ISBN: 3034808550
• ISBN13: 9783034808552
• Release Date: August 2014
• Publisher: Springer
• Length: 298 Pages
• Weight: 1.35 lbs.
• Dimensions: 0.8" x 6.1" x 9.2"