Flavins and Flavoproteins: Methods and Protocols

Book Overview

Part I: Riboflavin Production and Export by Microorganisms

1. Selection of Riboflavin Overproducing Strains of Lactic Acid Bacteria and Riboflavin Direct Quantification by Fluorescence

Pasquale Russo, Nicola De Simone, Vittorio Capozzi, Mari Luz Mohedano, Jos? ?ngel Ruiz-Mas?, Gloria Del Solar, Paloma L?pez, and Giuseppe Spano

2. Recent Advances in Construction of the Efficient Producers of Riboflavin and Flavin Nucleotides (FMN, FAD) in the Yeast Candida Famata

Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny

3. Overexpression of Riboflavin Excretase Enhances Riboflavin Production in the Yeast Candida Famata

Andriy O. Tsyrulnyk, Dariya V. Fedorovych, Kostyantyn V. Dmytruk, and Andriy A. Sibirny

Part II: Measuring Riboflavin Transport and Flavin Cofactor Metabolism with Recombinant Human Proteins

4. Functional Study of the Human Riboflavin Transporter 2 Using Proteoliposomes System

Lara Console, Maria Tolomeo, and Cesare Indiveri

5. Heterologous Over-Expression of Human FAD Synthase Isoforms 1 and 2

Michele Galluccio and Cesare Indiveri

6. Purification of Recombinant Human 6His-FAD Synthase (Isoform 2) and Quantitation of FAD / Protein Monomer Ratio by UV/Vis Spectra

Piero Leone, Stefano Quarta, Maria Tolomeo, and Maria Barile

7. Continuous and Discontinuous Approaches to Study FAD Synthesis and Degradation Catalyzed by Purified Recombinant FAD Synthase or Cellular Fractions

Piero Leone, Maria Tolomeo, and Maria Barile

Part III: Looking at Flavin Cofactors in Flavoenzymes

8. Redox Titration of Flavoproteins: An Overview

Francesco Bonomi and Stefania Iametti

9. Anaerobic Stopped-Flow Spectrophotometry with Photodiode Array Detection in the Pre-Steady State: An Application to Elucidate Oxido-Reduction Mechanisms in Flavoproteins

Patricia Ferreira and Milagros Medina

10. Atomic Force Microscopy: Single Molecule Imaging and Force Spectroscopy in the Study of Flavoproteins Ligand Binding and Reaction Mechanisms

Anabel Lostao and Milagros Medina

11. Ligand Binding in Allosteric Flavoproteins: Part 1. Quantitative Analysis of the Interaction with NAD+ of the Apoptosis Inducing Factor (AIF) Harboring FAD in the Reduced State

Paolo Cocomazzi, Luca Sorrentino, Federica Cossu, and Alessandro Aliverti

12. Ligand Binding in Allosteric Flavoproteins: Part 2. Quantitative Analysis of the Redox-Dependent Interaction of the Apoptosis-Inducing Factor (AIF) with Its Protein Partner

Paolo Cocomazzi, Delia Tarantino, Eloise Mastrangelo, and Alessandro Aliverti

13. Using D- and L-Amino Acid Oxidases to Generate the Imino Acid Substrate to Measure the Activity

• Format: Paperback
• Language: English
• ISBN: 1071612883
• ISBN13: 9781071612880
• Release Date: March 2022
• Publisher: Springer
• Length: 298 Pages
• Weight: 1.21 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"