Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing

Book Overview

The High Throughput Sequencing technologies triple-W discussion: Why to use HTS, What is the optimal HTS method to use, Which data analysis workflow to follow.- Whole genome sequencing recommendations.- Counseling for targeted DNA region re-sequencing.- Transcriptome profiling strategies.- Differential mRNA Alternative Splicing.- microRNA discovery and expression analysis in animals.- Analysis of Long Non-coding RNAs in RNA-seq data.- Ribosome profiling.- Genome-wide analysis of DNA methylation patterns by high-throughput sequencing.- Characterization of DNA-protein interactions: Design and analysis of ChIP-seq experiments.- PAR-CLIP: A genomic technique to dissect RNA-protein interactions.- Metagenomic design and sequencing.- A hitchhiker's guide to Metatranscriptomics.- Eukaryotic Single-Cell mRNA Sequencing.- Eukaryotic Single-Cell DNA Sequencing.- Submitting data to a public repository, the final step of a successful HTS experiment.

• Format: Hardcover
• Language: English
• ISBN: 3319313487
• ISBN13: 9783319313481
• Release Date: June 2016
• Publisher: Springer
• Length: 399 Pages
• Weight: 1.66 lbs.
• Dimensions: 0.9" x 6.1" x 9.2"