Experimental Models of Parkinson's Disease

Book Overview

Part I: Biochemical Experiments and Cellular Models of Parkinson's Disease

1. α-Synuclein Seeding Assay Using RT-QuIC

Ayami Okuzumi, Taku Hatano, Takeshi Fukuhara, Shinichi Ueno, Nobuyuki Nukina, Yuzuru Imai, and Nobutaka Hattori

2. Electron-Microscopic Analysis of α-Synuclein Fibrils

Airi Tarutani and Masato Hasegawa

3. α-Synuclein Seeding Assay Using Cultured Cells

Jun Ogata, Daisaku Takemoto, Shotaro Shimonaka, Yuzuru Imai, and Nobutaka Hattori

4. Analysis of α-Synuclein in Exosomes

Taiji Tsunemi, Yuta Ishiguro, Asako Yoroisaka, and Nobutaka Hattori

5. Measurement of GCase Activity in Cultured Cells

Yuri Shojima, Jun Ogata, Taiji Tsunemi, Yuzuru Imai, and Nobutaka Hattori

6. Detection of Substrate Phosphorylation of LRRK2 in Tissues and Cultured Cells

Kyohei Ito, Lejia Xu, Genta Ito, and Taisuke Tomita

7. Two Methods to Analyze LRRK2 Functions Under Lysosomal Stress: The Measurements of Cathepsin Release and Lysosomal Enlargement

Maria Sakurai and Tomoki Kuwahara

8. Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells

Kei-ichi Ishikawa, Risa Nonaka, and Wado Akamatsu

9. Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells

Kahori Shiba-Fukushima and Yuzuru Imai

Part II: Mammalian Models of Parkinson's Disease

10. Generation of Mitochondrial Toxin Rodent Models of Parkinson's Disease Using 6-OHDA, MPTP, and Rotenone

Hiroharu Maegawa and Hitoshi Niwa

11. Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson's Disease

Yuji Kamikubo, Keiko Wakisaka, Yuzuru Imai, and Takashi Sakurai

12. α-Synuclein Propagation Mouse Models of Parkinson's Disease

Norihito Uemura, Jun Ueda, Shinya Okuda, Masanori Sawamura, and Ryosuke Takahashi

13. Common Marmoset Model of α-Synuclein Propagation

Masami Masuda-Suzukake, Aki Shimozawa, Masashi Hashimoto, and Masato Hasegawa

14. Application of a Tissue Clearing Method for the Analysis of Dopaminergic Axonal Projections

Kenta Yamauchi, Megumu Takahashi, and Hiroyuki Hioki

15. Deep Brain Stimulation Using Animal Models of Parkinson's Disease

Asuka Nakajima and Yasushi Shimo

Part III: Invertebrate Models of Parkinson's Disease

16. Assessment of Cytotoxicity of α-Synuclein in Budding Yeast Using a Spot Growth Assay and Fluorescent Microscopy

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• Format: Paperback
• Language: English
• ISBN: 1071614975
• ISBN13: 9781071614976
• Release Date: May 2022
• Publisher: Humana
• Length: 217 Pages
• Weight: 0.90 lbs.
• Dimensions: 0.5" x 7.0" x 10.0"