Erk Signaling: Methods and Protocols

Book Overview

1. How Genetics has Helped Piece Together the MAPK Signaling Pathway
Dariel Ashton-Beaucage and Marc Therrien

2. In Vitro Enzyme Kinetics Analysis of EGFR
Zhihong Wang and Christine Candelora

3. High-Throughput Analysis of Mammalian Receptor Tyrosine Kinase Activation in Yeast Cells
Nobuo Yoshimoto and Shun'ichi Kuroda

4. Structural Studies of ERK2 Protein Complexes
Johannes F. Weijman, Stefan J. Riedl, and Peter D. Mace

5. Isolation and Characterization of Intrinsically Active (MEK-Independent) Mutants of Mpk1/Erk
Tal Goshen-Lago, Dganit Melamed, Arie Admon, and David Engelberg

6. Assaying Activation and Subcellular Localization of ERK in Cells and Tissues
Carme Caelles, Carles Bayod, and Melisa Morcillo

7. Detection and Functional Analysis of SUMO-Modified MEK
Yuji Kubota and Mutsuhiro Takekawa

8. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag
Liu Yang and Alexey Veraksa

9. High-Throughput In Vitro Identification of Direct MAPK/Erk Substrates
Rona Grossman and Ze'ev Paroush

10. Global Identification of ERK Substrates by Phosphoproteomics Based on IMAC and 2D-DIGE
Hidetaka Kosako and Kou Motani

11. Analysis of Ras/ERK Compartmentalization by Subcellular Fractionation
Lorena Agudo-Iba?ez, Piero Crespo, and Berta Casar

12. Cell-Based Assays to Study ERK Pathway/Caveolin1 Interactions
Raffaele Strippoli, Asier Echarri, Miguel Angel del Pozo

13. The Nuclear Translocation of ERK
Denise A. Berti and Rony Seger

14. Visualization of RAS/MAPK Signaling In Situ by the Proximity Ligation Assay (PLA)
Zijian Tang and Chengkai Dai

15. Measuring ERK Activity Dynamics in Single Living Cells Using FRET Biosensors
Yannick Blum, Rafael D. Fritz, Hyunryul Ryu, and Olivier Pertz

16. Quantifying Tensile Force and ERK Phosphorylation on Actin Stress Fibers
Hiroaki Hirata, Mukund Gupta, Sri Ram Krishna Vedula, Chwee Teck Lim, Benoit Ladoux, and Masahiro Sokabe

17. Co-Culture Activation of MAP Kinase in Drosophila S2 Cells
Josefa Steinhauer

18. Isolation of Mouse Embryonic Stem Cell Lines in the Study of ERK1/2 MAP Kinase Signaling
Marc K. Saba-El-Leil, Christophe Fr?min, and Sylvain Meloche

19. 3D Organotypic Culture Model to Study Components of ERK Signaling
Athina-Myrto Chioni, Rabia Tayba Bajwa, and Richard Grose

20. Genetic Validation of Cell Proliferation via Ras-Independent Activation of the Raf/Mek/Erk Pathway
Carmen G. Lechuga, Luc?a Sim?n-Carrasco, Harrys K.C. Jacob, and Matthias Drosten

21. Genome-Wide Analysis of RAS/ERK Signaling Targets
Joshua P. Plotnik and Peter C. Hollenhorst

22. Probing Chromatin Modifications in Response to ERK Signaling
Ozgur Oksuz and Wee-Wei Tee

23. Analyzing pERK Activation during Planarian Regeneration
Susanna Fraguas, Yoshihiko Umesono, Kiyokazu Agata, and Francesc Cebri?
24. Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development
Jessica Jie Chen and Swathi Arur

25. Reconstructing ERK Signaling in the Drosophila Embryo from Fixed Images
Bomyi Lim, Carmeline J. Dsilva, Ioannis G. Kevrekidis, and Stanislav Y. Shvartsman

• Format: Paperback
• Language: English
• ISBN: 1493981951
• ISBN13: 9781493981953
• Release Date: July 2018
• Publisher: Humana
• Length: 440 Pages
• Weight: 1.72 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"