DNA Electrophoresis: Methods and Protocols

Book Overview

1. DNA Electrophoresis: Historical and Theoretical Perspectives

Gareth A. Roberts and David T.F. Dryden

2. Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners

Svetlana Makovets

3. Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis

Nicholas P. Robinson

4. Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis

Andreas S. Ivessa

5. Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis

Andreas S. Ivessa

6. Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates

Jessica Lao, Shangming Tang and Neil Hunter

7. Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis

Jorge B. Schvartzman, Mar?a-Luisa Mart?nez-Robles, Pablo Hern?ndez, and Dora B. Krimer

8. A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks

Brian Pachkowski and Jun Nakamura

9. Denaturing Gradient Gel Electrophoresis (DGGE)

Fiona Strathdee and Andrew Free

10. Polyacrylamide Temperature Gradient Gel Electrophoresis

Viktor Viglasky

11. Separation of DNA Oligonucleotides using Denaturing Urea-PAGE

Fiona Flett and Heidrun Interthal

12. Pulsed-field Gel Electrophoresis of Bacterial Chromosomes

Julia S. P. Mawer and David R. F. Leach

13. Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis

Aziz El Hage and Jonathan Houseley

14. Analysis of DNA Damage Via Single Cell Electrophoresis

Diana Anderson and Julian Laubenthal

15. Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage

Julian Laubenthal and Diana Anderson

16. Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays

Lynn Powell

17. Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis

Tanya L. Williams and Daniel L. Levy

18. DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool

Fenfei Leng

19. Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity

Laura Gardano

• Format: Paperback
• Language: English
• ISBN: 1493959980
• ISBN13: 9781493959983
• Release Date: August 2016
• Publisher: Humana
• Length: 299 Pages
• Weight: 1.20 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"