Decellularization Methods of Tissue and Whole Organ in Tissue Engineering

Preface

Acknowledgment

Section 1: Introduction of Acellularization

Section 2: Extra Cellular Matrix (ECM) as a Bioscaffold

Section 3: Methods of Characterization of Acellularized Tissue

Histology Staining: H&E, Masson's Trichrome Staining, DAPI Staining

Determination amount of Hydroxy lysin and Hydroxy Proline

DNA Quantification

Scanning Electron Microscopy (SEM)

Mechanical Strength (Tensile and Compressive test)

Sodium Dodecyl Sulfate (SDS) Detection Method

Cytotoxicity Assessment: Live/Dead Assay, MTT Assay

Cell Attachment and Cell Proliferation

CT Angiography

Section 4: Methods of Sterilization of Acellularized Tissue

Section 5: skin

Chapter 1: Thin Skin

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Chapter 2: Thick Skin

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Section 6: Cartilage Tissue

Chapter 1: Hyaline cartilage

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Chapter 2: Fibrocartilage

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Chapter 3: Elastic cartilage

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Section 7: Bone Tissue

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Section 8: Cardiovascular System

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Section 9: Digestive System I

Chapter 1: Tongue

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Chapter 2: Esophagus

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

Chapter 3: Stomach

History

Materials & Methods

Cell seeding

Clinical Applications

Limitations

Conclusion

• Format: Hardcover
• Language: English
• ISBN: 3030827348
• ISBN13: 9783030827342
• Release Date: September 2021
• Publisher: Springer
• Length: 259 Pages
• Weight: 1.54 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"