Deamidation and Isoaspartate Formation in Peptides and Proteins (Advances in Analytical Biotechnology)

By Dana W. Aswad

As the biotechnology industry provides an increasing number of protein-based pharmaceuticals, it is important to understand the sources of heterogeneity and instability of purified proteins. Deamidation and Isoaspartate Formation in Peptides and Proteins focuses exclusively on these topics. It provides a comprehensive presentation of the mechanisms and conditions that lead to deamidation and isoaspartate formation, the effect of protein structure on the susceptibility of proteins to these reactions, the functional consequences of these reactions, and methodology that can be used to assess the amount and location of these damage reactions in proteins. The thirteen chapters, all written by highly respected experts, cover general reviews, case studies, and methodology. Numerous tables throughout the text provide quick access to precise data. For example, Table 1 in Chapter 13-Proteins that Deamidate Nonenzymatically-provides a significant source of protein information due to the endless research and dedication of its author, H.T. Wright. The information in this invaluable book is useful in evaluating alternative purification procedures, analyzing product purity, optimizing formulation and storage conditions, and designing modified proteins with altered stability characteristics.

• Format: Hardcover
• Language: English
• ISBN: 0849378230
• ISBN13: 9780849378232
• Release Date: November 1994
• Publisher: CRC Press
• Length: 272 Pages
• Weight: 1.28 lbs.
• Dimensions: 9.8" x 0.8" x 6.8"