Cystic Fibrosis: Diagnosis and Protocols, Volume 2: Methods and Resources to Understand Cystic Fibrosis

Book Overview

Part I: Pathophysiology of Cystic Fibrosis

1. Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology

Mark T. Clunes and Richard C. Boucher

2. Imaging CFTR Protein Localization in Cultured Cells and Tissues

Silvia M. Kreda and Martina Gentzsch

3. CFTR Regulation of Epithelial Sodium Channel

Yawar J. Qadri, Estelle Cormet-Boyaka, Dale J. Benos, and Bakhrom K. Berdiev

4. Methods for Evaluating Inflammation in Cystic Fibrosis

Assem G. Ziady and Pamela B. Davis

5. Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures

Erin N. Worthington and Robert Tarran

6. Measurement of Fluid Secretion from Intact Airway Submucosal Glands

Jeffrey J. Wine, Nam Soo Joo, Jae Young Choi, Hyungju Cho, Mauri E. Krouse, Jin V. Wu, Monal Khansaheb, Toshiya Irokawa, Juan Ianowski, John W. Hanrahan, Alan W. Cuthbert, and Kim V. Tran

7. Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia

Carla M.P. Ribeiro

8. Identification and Quantification of Mucin Expression

Kristina A. Thomsson and Gunnar C. Hansson

9. Methods to Classify Bacterial Pathogens in Cystic Fibrosis

Thomas Bjarnsholt, Xiaohui Chen Nielsen, Ulla Johansen, Lena N rgaard, and Niels H iby

10. Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells

Sophie Crespin, Marc Bacchetta, Song Huang, Tecla Dudez, Ludovic Wiszniewski, and Marc Chanson

Part II: Omic Approaches to Study Cystic Fibrosis

11. Introduction to Part II: Omics in the Biology of Cystic Fibrosis

William E. Balch

12. Microarray mRNA Expression Profiling to Study Cystic Fibrosis

Shyam Ramachandran, Luka A. Clarke, Todd E. Scheetz, Margarida D. Amaral, and Paul B. McCray, Jr.

13. Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture)

Ida Chiara Guerrera, Mario Ollero, Diane-Lore Vieu, and Aleksander Edelman

14. Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis

William E. Balch and John R. Yates III

15. Functional Genomics Assays to Study CFTR Traffic and ENaC Function

Joana Alma?a, Shehrazade Dahim?ne, Nicole Appel, Christian Conrad, Karl Kunzelmann, Rainer Pepperkok, and Margarida D. Amaral

16. New Lipidomic Approaches in Cystic Fibrosis

Mario Ollero, Ida Chiara Guerrera, Giuseppe Astarita, Daniele Piomelli, and Aleksander Edelman

Part III: Resources

17. Introduction to Part III: Resources for CFTR Research

Margarida D. Amaral

18. Primary Epithelial Cell Models for Cystic Fibrosis Research

Scott H. Randell, M. Leslie Fulcher, Wanda O'Neal, and John C. Olsen

19. Comparative Biology of Cystic Fibrosis Animal Models

John T. Fisher, Yulong Zhang, and John F. Engelhardt

20. CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction

Kathryn W. Peters, Tsukasa Okiyoneda, William E. Balch, Ineke Braakman, Jeffrey L. Brodsky, William B. Guggino, Christopher M.

• Format: Paperback
• Language: English
• ISBN: 1493962825
• ISBN13: 9781493962822
• Release Date: August 2016
• Publisher: Humana
• Length: 384 Pages
• Weight: 1.52 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"