Cyclic Nucleotide Signaling in Plants: Methods and Protocols

Book Overview

1: Molecular Methods for the Study of Signal Transduction in Plants

Helen R. Irving and Chris Gehring

2: Recombinant Expression and Functional Testing of Candidate Adenylate Cyclase Domains

Oziniel Ruzvidzo, Bridget T. Dikobe, David T. Kawadza, Grace H. Mabadahanye, Patience Chatukuta, Lusisizwe Kwezi

3: Quantification of Cyclic Dinucleotides by Reversed-Phase LC-MS/MS

Heike Burhenne and Volkhard Kaever

4: Determination of ADP-ribosyl Cyclase Activity, Cyclic ADP-ribose and Nicotinamide Adenine Dinucleotide Phosphate in Tissue Extracts

Richard M. Graeff and Hon Cheung Lee

5: In vivo Imaging of cGMP in Plants

Jean-Charles Isner and Frans J.M. Maathuis

6: Characterization of Heterologously Expressed Transporter Genes by Patch- and Voltage-Clamp Methods: Application to Cyclic Nucleotide-Dependent Responses

Fouad Lemtiri-Chlieh and Rashid Ali

7: Non-invasive Microelectrode Ion Flux Estimation Technique (MIFE) for the Study of the Regulation of Root Membrane Transport by Cyclic Nucleotides

Natalia Maria Ordo?ez, Lana Shabala, Chris Gehring, and Sergey Shabala

8: Calcium Imaging of the Cyclic Nucleotide Response

Martin R. McAinsh, Stephen K. Roberts and Lyudmila V. Dubovskaya

9: Identification and Quantification of Signal Molecule-dependent Protein Phosphorylation

Arnoud Groen, Ludivine Thomas, Kathryn Lilley and Claudius Marondedze

10: Comparative Gel-based (Phospho)Proteomics in Response to Signaling Molecules

Claudius Marondedze, Kathryn Lilley and Ludivine Thomas

11: An Affinity Pull-down Approach to Identify the Plant Cyclic Nucleotide Interactome

Lara Donaldson and Stuart Meier

12: Structural and Functional Characterization of Receptor Kinases with Nucleotide Cyclase Activity

Victor Muleya, Janet I. Wheeler and Helen R. Irving

13: Computational Identification of Candidate Nucleotide Cyclases in Higher Plants

Aloysius Wong and Chris Gehring

14: Identification of Cyclic Nucleotide Gated Channels Using Regular Expressions

Alice K. Zelman, Adam Dawe and Gerald A. Berkowitz

15: Inferring biological functions of guanylyl cyclases with computational methods

May Alqurashi and Stuart Meier

16: Identification and Characterization of Cyclic Nucleotide Phosphodiesterases

Erin B. Purcell and Rita Tamayo

17: Detection of reactive oxygen species down-stream of cyclic nucleotide signals in plants

Robin K. Walker and Gerald A. Berkowitz

18: Measurement of Nitric Oxide in Plant Tissue using Difluorofluorescein and Oxyhaemoglobin

Ndiko Ludidi

19: Infra-red Gas Analysis (IRGA) Technique for the Study of the Regulation of Photosynthetic Responses

Alex Valentine, Oziniel Ruzvidzo, Aleysia Kleinert, Yun Kang and Vagner Bennedito

• Format: Paperback
• Language: English
• ISBN: 1493962914
• ISBN13: 9781493962914
• Release Date: August 2016
• Publisher: Humana
• Length: 273 Pages
• Weight: 0.88 lbs.
• Dimensions: 0.6" x 6.1" x 9.2"