Claudins: Methods and Protocols

Book Overview

1. Measuring Size-Dependent Permeability of the Tight Junction Using PEG Profiling

Christina M. Van Itallie and James M. Anderson

2. Biochemical Analysis of Claudin Binding Compatibility

Christina Ward and Michael Koval

3. Electrophysiological Characterization of Claudin Ion Permeability Using Stably Transfected Epithelial Cell Lines

Alan S.L. Yu

4. The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its Status

Makoto Osanai

5. Interactions between Clostridium perfringens Enterotoxin and Claudins

Susan L. Robertson and Bruce A. McClane

6. Biophysical Methods to Probe Claudin Mediated Adhesion at the Cellular and Molecular Level

Sri Ram Krishna Vedula, Tong Seng Lim, Walter Hunziker, and Chwee Teck Lim

7. Detection of Tight Junction Barrier Function In Vivo by Biotin

Lei Ding, Yuguo Zhang, Rodney Tatum, and Yan-Hua Chen

8. The Co-Culture Method to Examine Interactions between Claudin Isoforms in Tight Junction-Free HEK293 Cells and Tight Junction-Bearing MDCK II Cells

Tetsuichiro Inai

9. Claudin 4: Functional Studies Beyond the Tight Junction

Holly A. Eckelhoefer, Thejani E. Rajapaksa, Jing Wang, Mary Stover-Hamer, Nancy C. Appleby, Jun Ling, and David D. Lo

10. Methods to Analyze Subcellular Localization and Intracellular Trafficking of Claudin 16

P. Jaya Kausalya and Walter Hunziker

11. Claudin Family Proteins in C. elegans

Jeffrey S.Simske and Jeff Hardin

12. In Vivo Imaging of Tight Junctions Using Claudin-EGFP Transgenic Medaka

Tatsuo Miyamoto, Mikio Furuse, and Makoto Furutani-Seiki

13. Claudins in a Primary Cultured Puffer Fish (Tetraodon nigroviridis) Gill Epithelium

Phuong Bui and Scott P. Kelly

14. Manipulating Claudin Expression in Avian Embryos

Michelle M. Collins and Aimee K. Ryan

15. Identification of Claudins by Western Blot and Immunofluorescence in Different Cell Lines and Tissues

Lorenza Gonz?lez-Mariscal, Erika Garay, and Miguel Quir?s

16. Expression and Function of Claudins in Hepatocytes

Takashi Kojima and Norimasa Sawada

17. Analysis of Changes in the Expression Pattern of Claudins Using Salivary Acinar Cells in Primary Culture

Junko Fujita-Yoshigaki

18. Development of Biological Tools to Study Claudins in the Male Reproductive Tract

Daniel G. Cyr, ?vemie Dub?, Julie Dufresne, and Mary Gregory

19. Using Molecular Tracers to Assess the Integrity of the Intestinal Epithelial Barrier In Vivo

Julian A. Guttman

20. Laboratory Methods in the Study of Endometrial Claudin-4

Paulo Serafini, Andr? Monteiro Rocha, Gary Daniel Smith, Eduardo Leme Alves da Motta, and Edmund Chada Baracat

21. Role of Claudins in Oxidant-Induced Alveolar Epithelial Barrier Dysfunction

Yu Sun, Richard D. Minshall, and Guochang Hu

22. Tracing the Endocytosis of Claudin-5 in Brain Endothelial Cells

Svetlana M. Stamatovic, Richard F. Keep, and Anuska V. Andjelkovic

23. Quantitative In Situ Analysis of Claudin Expression at the Blood-Retinal Barrier

• Format: Paperback
• Language: English
• ISBN: 1493958453
• ISBN13: 9781493958450
• Release Date: August 2016
• Publisher: Humana
• Length: 447 Pages
• Weight: 1.76 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"