Chromatin Remodeling: Methods and Protocols

Book Overview

1. Strain Construction and Screening Methods for a Yeast Histone H3/H4 Mutant Library

Junbiao Dai and Jef D. Boeke

2. Measuring Dynamic Changes in Histone Modifications and Nucleosome Density during Activated Transcription in Budding Yeast

Chhabi K. Govind, Daniel Ginsburg, and Alan G. Hinnebusch

3. Monitoring the Effects of Chromatin Remodelers on Long-Range Interactions In Vivo

Christine M. Kiefer and Ann Dean

4. Measuring Nucleosome Occupancy In Vivo by Micrococcal Nuclease

Gene O. Bryant

5. Analysis of Nucleosome Positioning Using a Nucleosome-Scanning Assay (NuSA)

Juan Jose Infante, G. Lynn Law, and Elton T. Young

6. Assaying Chromatin Structure and Remodeling by Restriction Enzyme Accessibility

Kevin W. Trotter and Trevor K. Archer

7. Generation of DNA Circles in Yeast by Inducible Site-Specific Recombination

Marc R. Gartenberg

8. An Efficient Purification System for Native Minichromosome from S. cerevisiae

Ashwin Unnikrishnan, Bungo Akiyoshi, Sue Biggins, and Toshio Tsukiyama

9. Simultaneous, Single-Molecule Detection of Endogenous C-5 DNA Methylation and Chromatin Accessibility Using MAPit

Russell P. Darst, Carolina E. Pardo, Santhi Pondugula, Vamsi K. Gangaraju, Nancy H. Nabilsi, Blaine Bartholomew, and Michael P. Kladde

10. Analysis of Stable and Transient Protein-Protein Interactions

Stephanie Byrum, Sherri K. Smart, Signe Larson, and Alan J. Tackett

11. Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Recovery after Photobleaching (FRAP)

Florian Mueller, Tatiana S. Karpova, Davide Mazza, and James G. McNally

12. Monitoring Dynamic Binding of Chromatin Proteins In Vivo by Fluorescence Correlation Spectroscopy (FCS) and Temporal Image Correlation Spectroscopy (TICS)

Davide Mazza, Timothy J. Stasevich, Tatiana S. Karpova, and James G. McNally

13. Analysis of Chromatin Structure in Plant Cells

Mala Singh, Amol Ranjan, Krishan Mohan Rai, Sunil Kumar Singh, Verandra Kumar, Ila Trivedi, Niraj Lodhi, and Samir V. Sawant

14. Analysis of Histones and Histone Variants in Plants

Ila Trivedi, Krishan Mohan Rai, Sunil Kumar Singh, Verandra Kumar, Mala Singh, Amol Ranjan, Niraj Lodhi, and Samir V. Sawant

15. Reconstitution of Modified Chromatin Templates for In Vitro Functional Assays

Miyong Yun, Chun Ruan, Jae-Wan Huh, and Bing Li

16. A Defined In Vitro System to Study ATP-Dependent Remodeling of Short Chromatin Fibers

Verena K. Maier and Peter B. Becker

17. In Vitro Reconstitution of In Vivo-Like Nucleosome Positioning on Yeast DNA

Christian J. Wippo and Philipp Korber

18. Activator-Dependent Acetylation of Chromatin Model Systems

Heather J. Szerlong and Jeffrey C. Hansen

19. Mapping Assembly-Favored and Remodeled Nucleosome Positions on Polynucleosomal Templates

Hillel I. Sims, Chuong Pham, and Gavin R. Schnitzler

20. Analysis of Changes in Nucleosome Conformation Using Fluorescence Resonance Energy Transfer (FRET)

Tina Shahian and Geeta J. Narlikar

21. Preparation of Nucleosomes Containing a Specific H2A-H2A Crosslink Forming a DNA-Constraining Loop Structure

Ning Liu and Jeffrey J. Hayes

• Format: Paperback
• Language: English
• ISBN: 1493962132
• ISBN13: 9781493962136
• Release Date: August 2016
• Publisher: Humana
• Length: 456 Pages
• Weight: 1.77 lbs.
• Dimensions: 0.9" x 7.0" x 10.0"