Chemical Neurobiology: Methods and Protocols

Book Overview

Part I. Chemical Probes of Membrane Protein Structure and Function

1. Engineering K+ Channels using Semisynthesis

Alexander G. Komarov, Corey A. Costantino, and Francis I. Valiyaveetil

2. Chemical Derivatization and Purification of Peptide-Toxins for Probing Ion Channel Complexes

Zhengmao Hua and William R. Kobertz

3. Using Yeast to Study Potassium Channel Function and Interactions with Small Molecules

Sviatoslav N. Bagriantsev and Daniel L. Minor Jr.

4. A FLIPR Assay for Evaluating Agonists and Antagonists of GPCR Heterodimers

Jessica H. Harvey, Richard M. van Rijn, and Jennifer L. Whistler

Part II. Photochemical Control of Protein and Cellular Function

5. Characterizing Caged Molecules Through Flash Photolysis and Transient Absorption Spectroscopy

Joseph P. Y. Kao and Sukumaran Muralidharan

6. Characterization of One- and Two- Photon Photochemical Uncaging Efficiency

Alexandre Specht, Frederic Bolze, Jean Francois Nicoud, and Maurice Goeldner

7. Photochromic Potassium Channel Blockers: Design and Electrophysiological Characterization

Alexandre Mourot, Timm Fehrentz, and Richard H. Kramer

8. A 1H NMR Assay for Measuring the Photostationary States of Photoswtichable Ligands

Matthew R. Banghart and Dirk Trauner

9. Developing a Photoreactive Antagonist

Pamela M. England

Part III. Chemical Probes for Imaging in the Nervous System

10. Development and in vitro Characterization of Ratiometric and Intensity-based Fluorescent Ion Sensors

Laurel A. Cooley, Vladimir V. Martin, and Kyle R. Gee

11. Characterization of Voltage-Sensitive Dyes in Living Cells using Two-Photon Excitation

Corey D. Acker and Leslie M. Loew

12. Characterization and Validation of Fluorescent Receptor Ligands: A Case Study of the Ionotropic Serotonin Receptor

Ruud Hovius

13. Imaging Single Synaptic Vesicles in Mammalian Central Synapses with Quantum Dots

Qi Zhang

14. Directed Evolution of Protein-Based Neurotransmitter Sensors for MRI

Philip A. Romero, Mikhail G. Shapiro, Frances H. Arnold, and Alan Jasanoff

• Format: Hardcover
• Language: English
• ISBN: 1627033440
• ISBN13: 9781627033442
• Release Date: March 2013
• Publisher: Humana
• Length: 202 Pages
• Weight: 1.31 lbs.
• Dimensions: 0.7" x 7.2" x 10.3"