Cardiac Gene Therapy: Methods and Protocols

Book Overview

Part I: Overview

1. Current Methods in Cardiac Gene Therapy: Overview

Kiyotake Ishikawa and Roger J. Hajjar

Part II: Genetic Modification

2. Silencing Genes in the Heart

Henry Fechner, Roland Vetter, Jens Kurreck, and Wolfgang Poller

3. Generation of Efficient miRNA Inhibitors Using Tough Decoy Constructs

Jimeen Yoo, Roger J. Hajjar, and Dongtak Jeong

4. Efficient Genome Editing in Induced Pluripotent Stem Cells with Engineered Nucleases In Vitro

Vittavat Termglinchan, Timon Seeger, Caressa Chen, Joseph C. Wu, and Ioannis Karakikes

5. Direct Cardiac Reprogramming as a Novel Therapeutic Strategy for Treatment of Myocardial Infarction

Hong Ma, Li Wang, Jiandong Liu, and Li Qian

Part III: Gene Delivery Vectors

6. Production and Characterization of Vectors Based on the Cardiotropic AAV Serotype 9

Erik Kohlbrenner and Thomas Weber

7. Cell-Based Measurement of Neutralizing Antibodies Against Adeno-Associated Virus (AAV)

Andreas Jungmann, Oliver M?ller, and Kleopatra Rapti

8. Synthesis of Modified mRNA for Myocardial Delivery

Jason Kondrat, Nishat Sultana, and Lior Zangi

9. Exosomes-Based Gene Therapy for MicroRNA Delivery

Prabhu Mathiyalagan and Susmita Sahoo

10. Lipidoid mRNA Nanoparticles for Myocardial Delivery in Rodents

Irene C. Turnbull, Ahmed A. Eltoukhy, Daniel G. Anderson, and Kevin D. Costa

Part IV: Gene Delivery Methods

11. Gene Transfer in Isolated Adult Cardiomyocytes

Kjetil Hodne, David B. Lipsett, and William E. Louch

12. Gene Transfer in Cardiomyocytes Derived from ES and iPS Cells

Francesca Stillitano, Ioannis Karakikes, and Roger J. Hajjar

13. Gene Transfer to Rodent Hearts In Vivo

Federica del Monte, Kiyotake Ishikawa, and Roger J. Hajjar

14. Ultrasound-Targeted Microbubble Destruction for Cardiac Gene Delivery

Shuyuan Chen and Paul A. Grayburn

15. A Needleless Liquid Jet Injection Delivery Approach for Cardiac Gene Therapy

Anthony S. Fargnoli, Michael G. Katz, and Charles R. Bridges

16. Cardiac Gene Delivery in Large Animal Models: Antegrade Techniques

Shin Watanabe, Lauren Leonardson, Roger J. Hajjar, and Kiyotake Ishikawa

17. Direct Myocardial Injection of Vectors

 

• Format: Paperback
• Language: English
• ISBN: 1493982435
• ISBN13: 9781493982431
• Release Date: July 2018
• Publisher: Humana
• Length: 355 Pages
• Weight: 1.40 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"