C. Elegans: Methods and Applications

1. Starting a C. elegans Research Laboratory: Practical Advice

Anne C. Hart and Heather. L. Bennett

2. The Basics of Setting Up Successful Teaching Labs and Short-Term Projects with C. elegans

Elizabeth E. Glater

3. Cryopreservation of C. elegans and Other Nematodes with Dimethyl Sulfoxide and Trehalose

Kevin F. O'Connell

4. Genetic Methods for Cellular Manipulation in C. elegans

Menachem Katz

5. Observing and Quantifying Fluorescent Reporters

Sreeparna Pradhan and Michael Hendricks

6. Microbial Rhodopsin Optogenetic Tools: Application for Analyses of Synaptic Transmission and of Neuronal Network Activity in Behavior

Amelie Bergs, Thilo Henss, Caspar Glock, Jatin Nagpal, and Alexander Gottschalk

7. Optogenetic Perturbation of Individual C. elegans Pharyngeal Neurons while Monitoring Feeding Behavior

Nicholas F. Trojanowski and Christopher Fang-Yen

8. Antibody Staining for Nematodes with Heat-Induced Antigen Retrieval (HIAR)

Curtis M. Loer

9. ExCel: Super-Resolution Imaging of C. elegans with Expansion Microscopy

Chih-Chieh (Jay) Yu, Danielle M. Orozco Cosio, and Edward S. Boyden

10. A Fusion-PCR Method for Expressing Genetic Tools in C. elegans

Eduard Bokman, Yifat Eliezer, and Alon Zaslaver

11. Approaches for CRISPR/Cas9 Genome Editing in C. elegans

Charlotte J. Martin and John A. Calarco

12. Targeted and Random Transposon-Assisted Single-Copy Transgene Insertion in C. elegans

Sonia El Mouridi and Christian Fr kj r-Jensen

13. Mutation Mapping and Identification by Whole-Genome Sequencing

Harold E. Smith

14. Lipid Extraction and Analysis

Henry H. Harrison and Jennifer L. Watts

15. Isolating Caenorhabditis elegans from the Natural Habitat

Clotilde Gimond, Nausicaa Poullet, and Christian Braendle

16. Microfluidic Devices for Behavioral Analysis, Microscopy, and Neuronal Imaging in Caenorhabditis elegans

Ross C. Lagoy, Eric Larsen, Dan Lawler, Hamilton White, and Dirk R. Albrecht

17. Neuronal Microsurgery with an Yb-Doped Fiber Femtosecond Laser

Maria B. Harreguy, Tracy S. Tran, and Gal Haspel

18. An Imaging System for Monitoring C. elegans Behavior and Aging

• Format: Hardcover
• Language: English
• ISBN: 1071621807
• ISBN13: 9781071621806
• Release Date: March 2022
• Publisher: Humana
• Length: 388 Pages
• Weight: 1.96 lbs.
• Dimensions: 1.0" x 7.1" x 10.0"