Biotechnology of Plant Secondary Metabolism: Methods and Protocols

Book Overview

1. Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal
Ganeshan Sivanandhan, Natesan Selvaraj, Andy Ganapathi, and Markandan Manickavasagan
2. Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
Thanise Nogueira, F?ller, J?lio C?sar de Lima, Fernanco de Costa, Kelly C.S. Rodrigues-Corr?a, and Arthur G. Fett-Neto
3. A Modified Protocol for High Quality RNA Extraction from Oleoresin-Producing Adult Pines
J?lio C?sar de Lima, Thanise Nogueira F?ller, Fernanda de Costa, Kelly C.S. Rodrigues-Corr?a, and Arthur G. Fett-Neto

4. Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
Svend Roesen Madsen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

5. From Plant Extract to a cDNA-Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
Fernando de Costa, Carla J.S. Barber, Darwin W. Reed, and Patrick S. Covello

6. Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbillis) in Escherichia coli
Fl?via Camila Schimpl, Eduardo Kiyota, and Paulo Mazzafera

7. Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
Vishal Singh Negi and Dulal Borthakur

8. Production of Aromatic Plant Terpenoids in Recombinant Baker's Yeast
Anita Emmerstorfer and Harald Pichler

9. Purification of a Recombinant Polyhistidine-Tagged Glycosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
Fernanda de Costa, Carla J.S. Barber, Pareshkumar T. Pujara, Darwin W. Reed, and Patrick S. Covello

10. A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
Morten Egevang J rgensen, Hussam Hassan Nour-Eldin, and Barbara Ann Halkier

11. Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
M?rcia R. de Almeida and Martina V. Str?mvik

12. Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
In?s Carqueijeiro, Viviana Martins, Henrique Noronha, Hern?ni Ger?s, and Mariana Sottomayor

13. Protoplast Transformation as a Plant-Transferable Transient Expression System
Patr?cia Duarte, Diana Ribeiro, In?s Carqueijeiro, Sara Bettencourt, and Mariana Sottomayor

14. Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System
Basdeo Bhagwat, Ming Chi, Dianwei Han, Haifeng Tang, Guiliang Tang, and Yu Xiang

15. Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs
Ming Chi, Basdeo Bhagwat, Guiliang Tang, and Yu Xiang

• Format: Paperback
• Language: English
• ISBN: 1493980408
• ISBN13: 9781493980406
• Release Date: March 2018
• Publisher: Humana
• Length: 180 Pages
• Weight: 0.76 lbs.
• Dimensions: 0.4" x 7.0" x 10.0"