Bioreactors in Stem Cell Biology: Methods and Protocols

Book Overview

1. Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines

Ang?lique Schmid, Emanuel Kreidl, Martin Bertschinger, and Patrick Vetsch

2. Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor

Aysegul Inam, Ezgi Rojda Taymaz, Mehmet Emin Uslu, Baris Binay, and Irem Deniz

3. Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volumed Bioreactors for Cell Culturing

Elif Gencturk, Senol Mutlu, and Kutlu O. Ulgen

4. Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors

Marites T. Woon, Puspa R. Pandey, and Inbar Friedrich Ben-Nun

5. High Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors

Xiaohua Lei, Chiyuan Ma, Yujing Cao, Yue Xiong, Jian V. Zhang, and Enkui Duan

6. Integrating Human Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture

Valerie Ho, Gerine Tong, Alan Lam, Shaul Reuveny, and Steve Oh

7. Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor

Misha Teale, Valentin Jossen, Dieter Eibl, and Regine Eibl

8. Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor

Huanye Xu, Zhongxiao Cong, Yuanyuan Zhang, Wei Liu, Xiaojun Yan, and Yanan Du

9. Optimized Method to Improve Cell Activity in 3D Scaffolds under a Dual Real-Time Dynamic Bioreactor System

Flavia Pedrini, Moema A. Hausen, and Eliana A.R. Duek

10. In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor

Ziming Chen, Peilin Chen, Rui Ruan, and Minghao Zheng

11. Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor

Kamil Wierzchowski and Maciej Pilarek

12. Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor

Georgia Pennarossa, Matteo Ghiringhelli, Fulvio Gandolfi, and Tiziana A.L. Brevini

13. Bioreactor-Based De-Epithelialization of Long-Segment Tracheal Grafts

Alba E. Marin-Araujo, Siba Haykal, and Golnaz Karoubi

14. Production of Extracellular Vesicles Using a CELLine Adherent Bioreactor Flask

Anastasiia Artuyants, Vanessa Chang, Gabrielle Reshef, Cherie Blenkiron, Lawrence W. Chamley, Euphemia Leung, and Colin L. Hisey

15. Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors

• Format: Paperback
• Language: English
• ISBN: 1071620207
• ISBN13: 9781071620205
• Release Date: January 2023
• Publisher: Humana
• Length: 273 Pages
• Weight: 1.11 lbs.
• Dimensions: 0.6" x 7.0" x 10.0"