Bioconjugate Techniques

By Greg T. Hermanson

Book Overview

Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.

• Format: Paperback
• Language: English
• ISBN: 0123423368
• ISBN13: 9780123423368
• Release Date: January 1996
• Publisher: Academic Press
• Length: 785 Pages
• Weight: 3.10 lbs.
• Dimensions: 10.0" x 1.5" x 7.0"

Customer Reviews

Fantastic, easy to read book

I am always extremely leary of biochemistry and/or bioconjugate books because if you're looking for a specific answer, you're not going to find it in a book. Instead, you have to bother those with experience and learn from their techniques. HOWEVER, this book is an exception in the highest regard! I was able to easily derive a surface chemistry protocol in around 10 minutes, and it only took me that long because I was fascinated on how direct this book was. Basically here's what you get: 1) Easy to read/decipher information 2) Fantastic Index 3) Quick answers that I couldn't even find on Google Best of all, in my opinion, is that each section is not prefaced with the past history of who/what/where/when/why, which although sometimes interesting, gives you no assistance in the lab.

a good book in bioconjugate chemistry area

It is hard for a chemistry major student to switch to biochemistry area. When a chem student try to read the big biochemistry textbook, he will be dizzy because of so many reaction equations and biocircles. To his most surprise, those equations are quite different with what he learned in origanic lecture. What shall he begin to go into the new world? Read Bioconjugate Technique --- this book will give Refresh you!