The present work has been carried out to study certain biochemical, histological and thermodynamical properties of buffalo (Bof. bubalis) peritoneum. The biochemical parameters consisted of estimation of total lipid, cholesterol, phospholipids, triglyceride, free fatty acid, ATPases and trace metal elements viz; Calcium, Magnesium, Iron, Copper, Nickel, Cobalt, Chromium, Zinc, Manganese, Cadmium and Lead. Histological studies were mainly directed to scanning electron microscopic examination of bovine peritoneum. However, routine histological examination of peritoneum membrane stained with Hematoxyline and Eosine (HE) has been performed