Basic Cell Culture: A Practical Approach (The ^APractical Approach Series)

By J.M. Davis

Book Overview

The use of animal, including human, cell culture has expanded enormously during the last twenty years, with the new applications appearing all the time. This book guides the newcomer progressively through all those areas which are basic to the performance of cell culture. It will also prove useful to the experienced worker entering a new field or setting up a new laboratroy, and as a source of reference on basic techniques. The topics covered include setting up and equipping a cell culture laboratory, sterilization of fluids and equipment, culture media, basic culture technique and the maintenance of cell lines, primary culture and the isolation of new cell lines, specific cell types and their requirements, single cell cloning, quality control of cell lines and the prevention, detection, and cure of contamination.

• Format: Paperback
• Language: English
• ISBN: 0199634335
• ISBN13: 9780199634330
• Release Date: April 1995
• Publisher: Oxford University Press
• Length: 301 Pages
• Weight: 1.28 lbs.
• Dimensions: 9.3" x 0.7" x 6.2"

Customer Reviews

intro to cell culture

I found this book to be very helpful. The information is current and clearly presented. (other texts I purchased had more recent publication dates, but were clearly out-of-date in the techniques and equipment used).I run a high through-put cell culture lab (about 4000 T175 per week). I purchased the text mainly as a training aid. 50% of my staff 'person hours' come from part time college students who only stayed for one or 2 semesters, thus I am faced with having to train new staff often. This text gives a good, clear introduction to the basics of cell culture and sterile technique and significantly cut down on the amount of time it took me to train new staff. I made it required reading for all my staff!