Barley: Methods and Protocols

1. An Introduction to Barley: The Crop and the Model

Wendy A. Harwood

2. Mutation Breeding in Barley: Historical Overview

Ludmila Ohnoutkova

3. A Practical Guide to Barley Crossing

William T.B. Thomas, Hazel Bull, Allan Booth, Ruth Hamilton, Brian P. Forster, and Jerome D. Franckowiak

4. Barley Anther Culture

Ludmila Ohnoutkova, Tomas Vlcko, and Mentewab Ayalew

5. Isolated Microspore Culture in Barley

Patricio Esteves and Fran?ois J. Belzile

6. TILLING in Barley

Matthias Jost, Miriam Szurman-Zubrzycka, Katarzyna Gajek, Iwona Szarejko, and Nils Stein

7. Virus-Induced Gene Silencing (VIGS) for Functional Characterization of Disease Resistance Genes in Barley Seedlings

Lokanadha R. Gunupuru, Alexandre Perochon, Shahin S. Ali, Steven R. Scofield, and Fiona M. Doohan

8. Agrobacterium-Mediated Transformation of Barley Immature Embryos

Alison Hinchliffe and Wendy A. Harwood

9. Methods for the Simple and Reliable Assessment of Barley Sensitivity to Abiotic Stresses during Early Development

Agata Daszkowska-Golec, Anna Skubacz, Marzena Kurowska, Michal Slota, Dorota Swiergolik, and Iwona Szarejko

10. Preparation of Barley Roots for Histological, Structural, and Immunolocalization Studies Using Light and Electron Microscopy

Marek Marzec and Michael Melzer

11. Preparation of Barley Pollen Mother Cells for Confocal and Super Resolution Microscopy

Sybille Mittmann, Mikel Arrieta, Luke Ramsay, Robbie Waugh, and Isabelle Colas

12. Microarrays for High-Throughput Gene Expression Analysis of Barley

Jenny A. Morris and Pete E. Hedley

13. Genome Engineering Using TALENs

Goetz Hensel and Jochen Kumlehn

14. Creating Targeted Gene Knock-Outs in Barley Using CRISPR/Cas9

Tom Lawrenson and Wendy Harwood

15. Genotyping-by-Sequencing on the Ion Torrent Platform in Barley

Amina Abed, Ga?tan L?gar?, Sonia Pomerleau, J?r?me St-Cyr, Brian Boyle, and Fran?ois Belzile

16. DNA Methylation Analysis in Barley and Other Species with Large Genomes

Karolina Chwialkowska, Urszula Korotko, and Miroslaw Kwasniewski

17. High Resolution RT-PCR Analysis of Alternative Barley Transcripts

Craig G. Simpson, John Fuller, Paulo Rapazote-Flores, Claus-Dieter Mayer, Cristiane P.G. Calixto, Linda Milne, Pe

• Format: Hardcover
• Language: English
• ISBN: 1493989421
• ISBN13: 9781493989423
• Release Date: November 2018
• Publisher: Humana
• Length: 313 Pages
• Weight: 1.73 lbs.
• Dimensions: 0.8" x 7.0" x 10.0"