Bacillus Thuringiensis Biotechnology

Book Overview

Index Of Contributors.- Preface.- PART I BACILLUS THURINGIENSIS: AN ENVIRONMENTALLY SAFETY ALTERNATIVE.- 1 Discovery and description of Bacillus thuringiensis.- 1 .1 Introduction.- 1.2 A brief History and Discovery of Bacillus thuringiensis.- 1.3 A Description of B. thuringiensis.- 1.4 Conclusions.- References.- 2 Bacillus thuringiensis applications in agriculture.- 2.1 Introduction.- 2.2 Methods of application of Bt and its products in agriculture.- 2.3 Advantages of using Bt products over chemical agents in agricultural practices.- 2.4 Threat to continuous use of Bt as biological control agent.- 2.5 Strategies to ensure continuing use of Bt and its products in agriculture.- References.- 3 Risk Assessment of Bt Transgenic Crops.- 3.1 Introduction.- 3.2 Perceived risks with Bt transgenic crops.- 3.3 Risk analysis of Bt transgenic crops.- 3.4 Oversight of Bt Transgenic crops.- 3.5 Conclusion and Future Prospects.- References.- 4 Use and efficacy of Bt compared to less environmentally safe alternatives .- 4.1 Biopesticide Classification.- 4.2 Biopesticide Market Survey.- 4.3 Use of Bt in IPM.- 4.4 Use and Efficacy of Bt.- References.- 5 Protein Engineering of Bacillus thuringiensis δ-Endotoxins .- 5.1 Introduction.- 5.2 Protein Engineering of Bt δ-endotoxins.- 5.3 Conclusions.- 5.4 Perspectives.- References.- PART II GENETICS OF BACILLUS THURINGIENSIS.- 6 Evolution of the Bacillus cereus group.- 6.1 The Bacillus cereus group.- 6.2 B. cereus group phylogeny.- 6.3 Bacillus cereus group genome sequencing.- 6.4 Bacillus cereus group bacteria - pathogens and symbionts.- 6.5 What distinguishes the species in the B. cereus group?.- 6.6 Importance of plasmids to B. cereus group biology.- References.- 7 Bacillus thuringiensis genetics and phages - from transduction and sequencing to recombineering .- 7.1 Introduction: a dramatic story of the Bacillus cereus genetics.- 7.2 Phage-mediated gene transduction.- 7.3 Phage-mediated gene transduction in the B. cereus group.- 7.4 Recombineering perspectives for the B. cereus group.- References.- 8 Conjugation in Bacillus thuringiensis: insights into the plasmids exchange process.- 8.1 Introduction.- 8.2 Conjugation as a tool to understand the ecology of B. cereus group.- 8.3 Conclusion and Perspectives.- References.- 9 Shuttle vectors of Bacillus thuringiensis .- 9.1 Introduction.- 9.2 Plasmids in Bacillus thuringiensis.- 9.3 Shuttle vectors of Bacillus thuringiensis.- 9.4 Transformation of Bacillus thuringiensis.- References.- 10 Construction and application in plasmid vectors of Bacillus cereus group .- 10.1 Introduction.- 10.2 Shuttle plasmid vectors in the B. cereus group.- 10.3 Integration vectors in the B. cereus group.- 10.4 Resolution vectors in the B. cereus group.- 10.5 High-effective expression vectors in the B. cereus group.- 10.6 Conclusion.- References.- 11 Recombination in Bacillus thuringiensis.- 11.1 Introduction.- 11.2 Site Specific Recombination in B. thuringiensis.- 11.3 Homologous Recombination in B. thuringiensis.- 11.4 Conclusion.- References.- 12 Genetic improvement of Bt strains and development of novel biopesticides.- 12.1 Introduction.- 12.2 Discovery of new Bt strains harbouring novel cry genes.- 12.3 Engineering of novel Cry proteins.- 12.4 Construction of new Bt strains by conjugation.- 12.5 Expression of Bt cry genes in heterologous microbial hosts.- 12.6 Development of recombinant Bt strains.- 12.7 Development of asporogenic Bt strains.- 12.8 Concluding remarks.- References.- PART III BT AS BIOPESTICIDE: APPLICATIONS IN BIOTECHNOLOGY.- 13 Genetically Modified Bacillus thuringiensis Biopesticides .- 13.1 Introduction.- 13.2 Strategies for constructing genetically modified Bt strains for engineered biopesticide development.- 13.3 Bt engineered strains with high toxicity or broader insecticidal spectrum.- 13.4 Multifunctional Bt engineered strains.- 13.5 Genetically modified Bt strains with delayed pest resistance.- References.- 14 Bacillus thuringien

• Format: Paperback
• Language: English
• ISBN: 9400799500
• ISBN13: 9789400799509
• Release Date: April 2014
• Publisher: Springer
• Length: 392 Pages
• Weight: 1.25 lbs.
• Dimensions: 0.9" x 6.1" x 9.0"