Antibody Methods and Protocols

Book Overview

1. Antigen Presentation for the Generation of Binding Molecules

Hilmar Ebersbach, Gabriele Proetzel, and Chonghui Zhang

2. Recombinant Antibodies and In Vitro Selection Technologies

C. Ronald Geyer, John McCafferty, Stefan D?bel, Andrew R.M. Bradbury, and Sachdev S. Sidhu

3. Phage Display

Konstantin Petropoulos

4. Generation of Human Fab Libraries for Phage Display

Christoph Rader

5. Selection of Human Fab Libraries by Phage Display

Christoph Rader

6. Ribosome Display

George Thom and Maria Groves

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

Chonghui Zhang

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

E-Chiang Lee and Michael Owen

9. Production of Human or Humanized Antibodies in Mice

Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogn?

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schr?ml and Matthias Biehl

12. Temperature-Dependent Antibody Kinetics as a Tool in Antibody Lead Selection

Michael Schr?ml and Leopold von Proff

13. Determination of Antibody Glycosylation by Mass Spectrometry

Christiane J?ger, Claudia Ferrara, Pablo Uma?a, Anne Zeck, J?rg Thomas Regula, and Hans Koll

14. Cloning, Expression, and Purification of Monoclonal Antibodies in scFv-Fc Format

Jiahui Yang and Christoph Rader

15. PEGylation of Antibody Fragments for Half-Life Extension

Simona Jevsevar, Mateja Kusterle, and Maja Kenig

16. Bispecific Antibody Derivatives Based on Full-Length IgG Formats

Michael Grote, Alexander K. Haas, Christian Klein, Wolfgang Schaefer, and Ulrich Brinkmann

17. Generation of Fluorescent IgG Fusion Proteins in Mammalian Cells

Alexander K. Haas, Klaus Mayer, and Ulrich Brinkmann

18. Methods to Engineer and Identify IgG1 Variants with Improved FcRn Binding or Effector Function

Robert F. Kelley and Y. Gloria Meng

19. Class-Specific Effector Functions of Therapeutic Antibodies

Virginie Pascal, Brice Laffleur, and Michel Cogn?

Konstantin Petropoulos

4. Generation of Human Fab Libraries for Phage Display

Christoph Rader

5. Selection of Human Fab Libraries by Phage Display

Christoph Rader

6. Ribosome Display

George Thom and Maria Groves

7. Hybridoma Technology for the Generation of Monoclonal Antibodies

Chonghui Zhang

8. The Application of Transgenic Mice for Therapeutic Antibody Discovery

E-Chiang Lee and Michael Owen

9. Production of Human or Humanized Antibodies in Mice

Brice Laffleur, Virginie Pascal, Christophe Sirac, and Michel Cogn?

10. Immortalization of Human B Cells: Analysis of B Cell Repertoire and Production of Human Monoclonal Antibodies

Elisabetta Traggiai

11. Kinetic Screening in the Antibody Development Process

Michael Schr?ml and Matthias Biehl

12. Temperature

• Format: Paperback
• Language: English
• ISBN: 1493959301
• ISBN13: 9781493959303
• Release Date: August 2016
• Publisher: Humana
• Length: 325 Pages
• Weight: 1.30 lbs.
• Dimensions: 0.7" x 7.0" x 10.0"