Analyzing Microbes: Manual of Molecular Biology Techniques

Book Overview

Microbial DNA extraction, purification and quantitation.- Fluorescent based detection, quantitation and expression of wviral gene by qRT-PCR.- Restriction enzymes and their role in microbiology.- Genetic fingerprinting techniques for molecular characterization of microbes.- Agarose gel electrophoresis and Polyacrylamide agarose gel electrophoresis: Methods and principles.- Molecular identification of microbes.- Preservation and maintenance of microbial cultures.- Microbes from extreme environment: Molecular identification procedures.- ELISA based identification and detection of microbes.- Analysis of microbial diversity and construction of metagenomic library.- Bioinformatics tools for interpretation of data used in molecular identification.- Molecular phylogenetics of microbes.- Microarray technology: Basic concept, protocols and applications.- Microarray analysis of different functional genes of microorganisms.- DNA cloning and sequencing.- Biological sequence analysis: Algorithms and statistical methods.

• Format: Hardcover
• Language: English
• ISBN: 3642344097
• ISBN13: 9783642344091
• Release Date: February 2013
• Publisher: Springer
• Length: 352 Pages
• Weight: 1.78 lbs.
• Dimensions: 1.1" x 7.2" x 10.3"