A Practical Guide to Influenza Virus Isolation and Identification

Bys: Boora, Sanjit, Khan, Anish, Kaushik, Samander

This practical guide details the essential methodologies for the isolation, identification, and quantification of Influenza A viruses, specifically focusing on the H1N1pdm09 strain using MDCK cell culture systems. It provides a comprehensive workflow that includes standardized protocols for the maintenance and subculturing of MDCK cells, viral propagation techniques, and molecular confirmation via RT-PCR targeting the HA gene. Furthermore, the text offers in-depth instruction on viral quantification methods, employing Hemagglutination (HA) assays to determine total viral titers and TCID50 assays using the Reed-Muench method for calculating infectious titers. By combining theoretical background with rigorous experimental data and step-by-step procedural flowcharts, this work serves as a vital resource for researchers and laboratory professionals establishing robust platforms for influenza virus characterization and antiviral drug discovery.

• Format: Paperback
• Language: English
• ISBN: 6209309305
• ISBN13: 9786209309304
• Release Date: December 2025
• Publisher: LAP Lambert Academic Publishing
• Length: 52 Pages
• Weight: 0.18 lbs.
• Dimensions: 0.1" x 6.0" x 9.0"