The only text providing practical guidelines to a wide range of topics important to the practicing enzymologist. It offers advice on how solvents, salts, substrates, and denaturants affect enzyme function and stability; developing a scheme for purifying an enzyme from different kinds of tissue; applying different techniques for fractionating proteins; characterizing proteins by determining molecular weight, isoelectric pH, amino acid composition and sequence, ligand binding, and enzyme kinetics. Basic guidelines to procedures and hints for the operation of a technique are also included
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