Using Mass Spectrometry for Biochemical Studies on Enzymatic Domains from Polyketide Synthases

by Jenner Matthew and Matthew Jenner

Book Overview

This thesis reports studies on the substrate specificity of crucial ketosynthase (KS) domains from trans-AT Polyketide Synthases (PKSs). Using a combination of electrospray ionisation-mass spectrometry (ESI-MS) and simple N-acetyl cysteamine (SNAC) substrate mimics, the specificity of a range of KS domains from the bacillaene and psymberin PKSs have been succsessfully studied with regard to the initial acylation step of KS-catalysis.

In addition,...

• Format: Paperback
• Language: English
• ISBN: 3319813552
• ISBN13: 9783319813554
• Release Date: May 2018
• Publisher: Springer
• Length: 176 Pages
• Weight: 0.62 lbs.
• Dimensions: 0.4" x 6.1" x 9.2"